Inhibitory effect of ethanol on growth and solute accumulation by Saccharomyces cerevisiae as affected by plasma-membrane lipid composition - PubMed (original) (raw)

PMID: 391174
DOI: 10.1007/BF00408045

Inhibitory effect of ethanol on growth and solute accumulation by Saccharomyces cerevisiae as affected by plasma-membrane lipid composition

D S Thomas et al. Arch Microbiol. 1979 Jul.

Abstract

Incorporation of ethanol (1.0 or 1.25 M) into exponential-phase cultures of Saccharomyces cerevisiae NCYC 366 growing anaerobically in a medium supplemented with ergosterol and an unsaturated fatty acid caused a retardation in growth rat, which was greater when the medium contained oleic rather than linoleic acid. Ethanol incorporation led to an immediate drop in growth rate, and ethanol-containing cultures grew at the slower rate for at least 10 h. Incorporation of ethanol (0.5 M) into buffered (pH 4.5) cell suspensions containing D-[6-3H] glucose, D-[1-14C] glucosamine, L-[U-14C] lysine or arginine, or KH232PO4 lowered the rate of solute accumulation by cells. Rates of accumulation of glucose, lysine and arginine were retarded to a greater extent when cells had been grown in the presence of oleic rather than linoleic acid. This difference was not observed with accumulation of phosphate. Ethanol was extracted from exponential-phase cells by four different methods. Cells grown in the presence of linoleic acid contained a slightly, but consistently, lower concentration of ethanol than cells grown in oleic acid-containing medium. The ethanol concentration in cells was 5-7 times greater than that in the cell-free medium.

PubMed Disclaimer

Cited by

Responses of Issatchenkia terricola WJL-G4 upon Citric Acid Stress.
Liu X, Tang Y, Ning W, Bao Y, Luo T, Wang J. Liu X, et al. Molecules. 2022 Apr 21;27(9):2664. doi: 10.3390/molecules27092664. Molecules. 2022. PMID: 35566015 Free PMC article.
Accelerated Bioprocess Development of Endopolygalacturonase-Production with Saccharomyces cerevisiae Using Multivariate Prediction in a 48 Mini-Bioreactor Automated Platform.
Sawatzki A, Hans S, Narayanan H, Haby B, Krausch N, Sokolov M, Glauche F, Riedel SL, Neubauer P, Cruz Bournazou MN. Sawatzki A, et al. Bioengineering (Basel). 2018 Nov 21;5(4):101. doi: 10.3390/bioengineering5040101. Bioengineering (Basel). 2018. PMID: 30469407 Free PMC article.
Genetic Basis of Variation in Heat and Ethanol Tolerance in Saccharomyces cerevisiae.
Riles L, Fay JC. Riles L, et al. G3 (Bethesda). 2019 Jan 9;9(1):179-188. doi: 10.1534/g3.118.200566. G3 (Bethesda). 2019. PMID: 30459179 Free PMC article.
Examining the role of membrane lipid composition in determining the ethanol tolerance of Saccharomyces cerevisiae.
Henderson CM, Block DE. Henderson CM, et al. Appl Environ Microbiol. 2014 May;80(10):2966-72. doi: 10.1128/AEM.04151-13. Epub 2014 Mar 7. Appl Environ Microbiol. 2014. PMID: 24610851 Free PMC article.

References

1. J Cell Comp Physiol. 1953 Feb;41(1):23-36 - PubMed
1. Arch Microbiol. 1977 Aug 26;114(2):137-42 - PubMed
1. J Gen Microbiol. 1973 Aug;77(2):371-82 - PubMed
1. J Nutr. 1969 Jun;98(2):222-4 - PubMed
1. J Biol Chem. 1962 Jun;237:1793-800 - PubMed

Inhibitory effect of ethanol on growth and solute accumulation by Saccharomyces cerevisiae as affected by plasma-membrane lipid composition - PubMed (original) (raw)