Creutzfeldt-Jakob disease prion proteins in human brains - PubMed (original) (raw)

Case Reports

Creutzfeldt-Jakob disease prion proteins in human brains

J M Bockman et al. N Engl J Med. 1985.

Abstract

Creutzfeldt-Jakob disease is caused by a slow infectious pathogen, or prion. We found that purified fractions from the brains of two patients with Creutzfeldt-Jakob disease contained protease-resistant proteins ranging in apparent molecular weight from 10,000 to 50,000. These proteins reacted with antibodies raised against the scrapie prion protein PrP 27-30. Rod-shaped particles were found in the brain tissue of the patients that were similar to those isolated from rodents with either scrapie or experimental Creutzfeldt-Jakob disease. After being stained with Congo red dye, the protein polymers from patients with Creutzfeldt-Jakob disease exhibited green birefringence when examined under polarized light. Our findings suggest that the amyloid plaques found in the brains of patients with Creutzfeldt-Jakob disease may be composed of paracrystalline arrays of prions similar to those in prion diseases in laboratory animals.

PubMed Disclaimer

Cited by

Characterization of major peptides in Creutzfeldt-Jakob disease and scrapie.
Sklaviadis T, Manuelidis L, Manuelidis EE. Sklaviadis T, et al. Proc Natl Acad Sci U S A. 1986 Aug;83(16):6146-50. doi: 10.1073/pnas.83.16.6146. Proc Natl Acad Sci U S A. 1986. PMID: 3090551 Free PMC article.
Abnormal isoform of prion proteins accumulates in the synaptic structures of the central nervous system in patients with Creutzfeldt-Jakob disease.
Kitamoto T, Shin RW, Doh-ura K, Tomokane N, Miyazono M, Muramoto T, Tateishi J. Kitamoto T, et al. Am J Pathol. 1992 Jun;140(6):1285-94. Am J Pathol. 1992. PMID: 1351366 Free PMC article.
Prion liposomes.
Gabizon R, Prusiner SB. Gabizon R, et al. Biochem J. 1990 Feb 15;266(1):1-14. doi: 10.1042/bj2660001. Biochem J. 1990. PMID: 1968741 Free PMC article. Review. No abstract available.
Accumulation of abnormal prion protein in mice infected with Creutzfeldt-Jakob disease via intraperitoneal route: a sequential study.
Muramoto T, Kitamoto T, Tateishi J, Goto I. Muramoto T, et al. Am J Pathol. 1993 Nov;143(5):1470-9. Am J Pathol. 1993. PMID: 8238261 Free PMC article.
Scrapie and Creutzfeldt-Jakob disease prion proteins share physical properties and antigenic determinants.
Bendheim PE, Bockman JM, McKinley MP, Kingsbury DT, Prusiner SB. Bendheim PE, et al. Proc Natl Acad Sci U S A. 1985 Feb;82(4):997-1001. doi: 10.1073/pnas.82.4.997. Proc Natl Acad Sci U S A. 1985. PMID: 2579394 Free PMC article.

Creutzfeldt-Jakob disease prion proteins in human brains - PubMed (original) (raw)