Correction of a nucleotide-excision-repair mutation by human chromosome 19 in hamster-human hybrid cells - PubMed (original) (raw)

Comparative Study

Correction of a nucleotide-excision-repair mutation by human chromosome 19 in hamster-human hybrid cells

L H Thompson et al. Somat Cell Mol Genet. 1985 Jan.

Abstract

A UV-sensitive mutant line of CHO cells, UV20, was shown to be phenotypically corrected to resistance by fusion with human lymphocytes or fibroblasts. Only human chromosome 19 correlated with the DNA repair phenotype of resistant hybrid clones and their resistant or sensitive subclones. This study demonstrates the mapping of a human repair gene by direct selection of complementing hybrids in the presence of a DNA-damaging agent (mitomycin C).

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