Identification of a lymphokine that stimulates eosinophil differentiation in vitro. Its relationship to interleukin 3, and functional properties of eosinophils produced in cultures - PubMed (original) (raw)
Identification of a lymphokine that stimulates eosinophil differentiation in vitro. Its relationship to interleukin 3, and functional properties of eosinophils produced in cultures
C J Sanderson et al. J Exp Med. 1985.
Abstract
Factors stimulating eosinophil differentiation in vitro have been studied by means of a liquid bone marrow culture system in which the number of eosinophils is estimated directly by morphology or indirectly by assay for eosinophil peroxidase. The results show that eosinophil colonies are not formed in agar, emphasizing the importance of the liquid culture system. Three types of evidence identify a novel lymphokine, eosinophil-differentiating factor (EDF). (a) Coordinate analysis of lymphokine activity in media conditioned by a panel of parasite antigen and another panel of alloantigen-reactive T cell clones indicates that EDF is distinct from interleukin 2 (IL-2), IL-3, and bone marrow proliferation activity (BMPA). (b) A T hybrid (NIMP-TH1) produces EDF but no IL-2, IL-3, interferon, or colony-stimulating factor. (c) Gel filtration of conditioned media (CM) indicates that NIMP-TH1 and a T clone (NIMP-T2) produce EDF (Mr 46,000). NIMP-T2 also produced IL-3 (Mr 26,000) but this was easily separated from EDF. IL-3 is also shown to have eosinophil differentiation activity (EDA) but this represents a very small proportion of the EDA in T2-CM. Fractionation of WEHI-3-CM indicates that EDA from this source has a similar elution profile to IL-3 (Mr 35-36,000). Furthermore, a comparison of the relative activities in purified IL-3 and WEHI-3-CM indicates that all the EDA can be attributed to the IL-3 in the latter. EDF is shown to stimulate production of eosinophils in long-term bone marrow cultures; the kinetics of eosinophil production suggests that EDF is acting on committed precursors in the bone marrow. The transient nature of eosinophil production suggests that precursors from multipotential stem cells are not produced. The eosinophils produced in these cultures are morphologically normal and functional in that they lysed sheep red blood cells coated with IgG1, IgG2a, and IgG2b, but not with IgM, IgA, or IgE. In addition, they were capable of adhering to and killing Schistosoma mansoni schistosomula.
Similar articles
- Murine eosinophil differentiation factor. An eosinophil-specific colony-stimulating factor with activity for human cells.
Lopez AF, Begley CG, Williamson DJ, Warren DJ, Vadas MA, Sanderson CJ. Lopez AF, et al. J Exp Med. 1986 May 1;163(5):1085-99. doi: 10.1084/jem.163.5.1085. J Exp Med. 1986. PMID: 3486243 Free PMC article. - Production of a T-cell hybrid producing a lymphokine stimulating eosinophil differentiation.
Warren DJ, Sanderson CJ. Warren DJ, et al. Immunology. 1985 Apr;54(4):615-23. Immunology. 1985. PMID: 2579895 Free PMC article. - The production of lymphokines by primary alloreactive T-cell clones: a co-ordinate analysis of 233 clones in seven lymphokine assays.
Sanderson CJ, Strath M, Warren DJ, O'Garra A, Kirkwood TB. Sanderson CJ, et al. Immunology. 1985 Dec;56(4):575-84. Immunology. 1985. PMID: 3935571 Free PMC article. - Eosinophil differentiation factor (interleukin-5).
Sanderson CJ. Sanderson CJ. Immunol Ser. 1990;49:231-56. Immunol Ser. 1990. PMID: 2090253 Review.
Cited by
- Differential effects of short- and long-term treatment with mepolizumab on eosinophil kinetics in blood and sputum in eosinophilic asthma.
Hassani M, Tak T, van Aalst C, van Nederveen S, Tesselaar K, Vrisekoop N, Koenderman L. Hassani M, et al. iScience. 2021 Jul 28;24(8):102913. doi: 10.1016/j.isci.2021.102913. eCollection 2021 Aug 20. iScience. 2021. PMID: 34409272 Free PMC article. - Identification of a novel IL-5 signaling pathway in chronic pancreatitis and crosstalk with pancreatic tumor cells.
Gitto SB, Beardsley JM, Nakkina SP, Oyer JL, Cline KA, Litherland SA, Copik AJ, Khaled AS, Fanaian N, Arnoletti JP, Altomare DA. Gitto SB, et al. Cell Commun Signal. 2020 Jun 17;18(1):95. doi: 10.1186/s12964-020-00594-x. Cell Commun Signal. 2020. PMID: 32552827 Free PMC article. - Identification of a novel splice variant for mouse and human interleukin-5.
Shilovskiy I, Andreev S, Mazurov D, Barvinskaia E, Bolotova S, Nikolskii A, Sergeev I, Maerle A, Kudlay D, Khaitov M. Shilovskiy I, et al. Heliyon. 2020 Mar 18;6(3):e03586. doi: 10.1016/j.heliyon.2020.e03586. eCollection 2020 Mar. Heliyon. 2020. PMID: 32211550 Free PMC article. - Peripheral Innate Lymphoid Cells Are Increased in First Line Metastatic Colorectal Carcinoma Patients: A Negative Correlation With Th1 Immune Responses.
Loyon R, Jary M, Salomé B, Gomez-Cadena A, Galaine J, Kroemer M, Romero P, Trabanelli S, Adotévi O, Borg C, Jandus C. Loyon R, et al. Front Immunol. 2019 Sep 6;10:2121. doi: 10.3389/fimmu.2019.02121. eCollection 2019. Front Immunol. 2019. PMID: 31555301 Free PMC article. Clinical Trial. - Proinflammatory Pathways in the Pathogenesis of Asthma.
Peebles RS Jr, Aronica MA. Peebles RS Jr, et al. Clin Chest Med. 2019 Mar;40(1):29-50. doi: 10.1016/j.ccm.2018.10.014. Clin Chest Med. 2019. PMID: 30691715 Free PMC article. Review.
References
- J Exp Med. 1970 Jun 1;131(6):1288-305 - PubMed
- J Cell Physiol. 1974 Oct;84(2):275-89 - PubMed
- Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Dec;82(6):919-20 - PubMed
- Blood. 1976 May;47(5):757-65 - PubMed
- Nature. 1976 Jul 29;262(5567):397-9 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous