Nucleotide binding by membrane components of bacterial periplasmic binding protein-dependent transport systems - PubMed (original) (raw)
Comparative Study
Nucleotide binding by membrane components of bacterial periplasmic binding protein-dependent transport systems
C F Higgins et al. EMBO J. 1985 Apr.
Abstract
Bacterial periplasmic binding protein-dependent transport systems require the function of a specific substrate-binding protein, located in the periplasm, and several membrane-bound components. We present evidence for a nucleotide-binding site on one of the membrane components from each of three independent transport systems, the hisP, malK and oppD proteins of the histidine, maltose and oligopeptide permeases, respectively. The amino acid sequence of the oppD protein has been determined and this protein is shown to share extensive homology with the hisP and malK proteins. Three lines of evidence lead us to propose the existence of a nucleotide-binding site on each of these proteins. A consensus nucleotide-binding sequence can be identified in the same relative position in each of the three proteins. The oppD protein binds to a Cibacron Blue affinity column and can be eluted by ATP but not by CTP or NADH. The oppD protein is labelled specifically by the nucleotide affinity analogue 5'-p-fluorosulphonylbenzoyladenosine. The identification of a nucleotide-binding site provides strong evidence that transport by periplasmic binding protein-dependent systems is energized directly by the hydrolysis of ATP or a closely related nucleotide. The hisP, malK and oppD proteins are thus responsible for energy-coupling to their respective transport systems.
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References
- Nature. 1970 Aug 15;227(5259):680-5 - PubMed
- Proc Natl Acad Sci U S A. 1973 May;70(5):1514-8 - PubMed
- J Biol Chem. 1974 Jan 25;249(2):634-44 - PubMed
- J Biol Chem. 1974 Oct 10;249(19):6138-43 - PubMed
- J Biol Chem. 1974 Dec 25;249(24):7747-55 - PubMed
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