Effect of interleukin 2, interferon-gamma, and mitogens on the production of tumor necrosis factors alpha and beta - PubMed (original) (raw)

. 1985 Oct;135(4):2492-7.

• PMID: 3928750

Effect of interleukin 2, interferon-gamma, and mitogens on the production of tumor necrosis factors alpha and beta

G E Nedwin et al. J Immunol. 1985 Oct.

Abstract

Human peripheral blood mononuclear cells (PBMC) were induced by recombinant interleukin 2 and mitogens to secrete two distinct cytotoxic polypeptides, tumor necrosis factor-alpha (TNF-alpha) and tumor necrosis factor-beta (TNF-beta), previously called lymphotoxin. Treatment of PBMC with recombinant human interleukin 2 (rIL 2) or mitogens in combination with recombinant human interferon-gamma (rIFN-gamma) resulted in augmented production of both TNF-alpha and TNF-beta. rIFN-gamma alone had no effect on production of either cytotoxic polypeptide. TNF-alpha was produced within 2 to 3 hr after induction and was the major cytotoxin produced by PBMC during the first 48 hr of culture, after which time TNF-beta became the predominant species. TNF-beta was first secreted into the media after 8 hr of induction. Enhanced levels of both TNF-alpha and TNF-beta were seen when the PBMC were separated into adherent and nonadherent cells. Both TNF-alpha and TNF-beta were induced in different tumor cell lines of hematopoietic origin. The results demonstrate that the production of TNF-alpha and TNF-beta can be enhanced by two lymphokines, IL 2 and IFN-gamma.

PubMed Disclaimer

Similar articles

• Interferon-gamma enhances induction of lymphotoxin in recombinant interleukin 2-stimulated peripheral blood mononuclear cells.
  Svedersky LP, Nedwin GE, Goeddel DV, Palladino MA Jr. Svedersky LP, et al. J Immunol. 1985 Mar;134(3):1604-8. J Immunol. 1985. PMID: 3918106
• Natural killer-sensitive targets stimulate production of TNF-alpha but not TNF-beta (lymphotoxin) by highly purified human peripheral blood large granular lymphocytes.
  Peters PM, Ortaldo JR, Shalaby MR, Svedersky LP, Nedwin GE, Bringman TS, Hass PE, Aggarwal BB, Herberman RB, Goeddel DV, et al. Peters PM, et al. J Immunol. 1986 Oct 15;137(8):2592-8. J Immunol. 1986. PMID: 3760569
• Tumor necrosis factor and interleukin 1: cytokines with multiple overlapping biological activities.
  Le J, Vilcek J. Le J, et al. Lab Invest. 1987 Mar;56(3):234-48. Lab Invest. 1987. PMID: 3029503 Review. No abstract available.
• Interferon-gamma, more of a cachectin than tumor necrosis factor.
  Billiau A, Matthys P. Billiau A, et al. Cytokine. 1992 Jul;4(4):259-63. doi: 10.1016/1043-4666(92)90065-y. Cytokine. 1992. PMID: 1515549 Review. No abstract available.

Cited by

• Tumour necrosis factor gene polymorphisms in Egyptian patients with rheumatoid arthritis and their relation to disease activity and severity.
  Zaghlol HM, Abdelshafy S, Mohamed RA, Abdelaleem EA. Zaghlol HM, et al. Cent Eur J Immunol. 2019;44(3):277-284. doi: 10.5114/ceji.2019.89602. Epub 2019 Sep 30. Cent Eur J Immunol. 2019. PMID: 31933537 Free PMC article.
• Association of tumour necrosis factor-α (TNF-α) gene polymorphisms (-308 G>A and -238 G>A) and the risk of severe dengue: A meta-analysis and trial sequential analysis.
  Naing C, Htet NH, Siew Tung W, Basavaraj AK, Mak JW. Naing C, et al. PLoS One. 2018 Oct 9;13(10):e0205413. doi: 10.1371/journal.pone.0205413. eCollection 2018. PLoS One. 2018. PMID: 30300401 Free PMC article.
• Tumor necrosis factor-alpha (- 308G/A, + 488G/A, - 857C/T and -1031 T/C) gene polymorphisms and risk of ischemic stroke in north Indian population: A hospital based case-control study.
  Kumar P, Kumar A, Misra S, Sagar R, Faruq M, Suroliya V, Vivekanandhan S, Srivastava AK, Prasad K. Kumar P, et al. Meta Gene. 2015 Nov 17;7:34-9. doi: 10.1016/j.mgene.2015.11.003. eCollection 2016 Feb. Meta Gene. 2015. PMID: 26862479 Free PMC article.
• Clinical chorioamnionitis at term V: umbilical cord plasma cytokine profile in the context of a systemic maternal inflammatory response.
  Romero R, Chaemsaithong P, Docheva N, Korzeniewski SJ, Tarca AL, Bhatti G, Xu Z, Kusanovic JP, Chaiyasit N, Dong Z, Yoon BH, Hassan SS, Chaiworapongsa T, Yeo L, Kim YM. Romero R, et al. J Perinat Med. 2016 Jan;44(1):53-76. doi: 10.1515/jpm-2015-0121. J Perinat Med. 2016. PMID: 26360486 Free PMC article.
• Clinical chorioamnionitis at term IV: the maternal plasma cytokine profile.
  Romero R, Chaemsaithong P, Docheva N, Korzeniewski SJ, Tarca AL, Bhatti G, Xu Z, Kusanovic JP, Dong Z, Chaiyasit N, Ahmed AI, Yoon BH, Hassan SS, Chaiworapongsa T, Yeo L. Romero R, et al. J Perinat Med. 2016 Jan;44(1):77-98. doi: 10.1515/jpm-2015-0103. J Perinat Med. 2016. PMID: 26352068 Free PMC article.

MeSH terms

Substances

LinkOut - more resources

• Other Literature Sources

  • The Lens - Patent Citations