Deletions of kappa chain constant region genes in mouse lambda chain-producing B cells involve intrachromosomal DNA recombinations similar to V-J joining - PubMed (original) (raw)

Deletions of kappa chain constant region genes in mouse lambda chain-producing B cells involve intrachromosomal DNA recombinations similar to V-J joining

M W Moore et al. Proc Natl Acad Sci U S A. 1985 Sep.

Abstract

We isolated and characterized the germ-line counterpart of a DNA segment designated RS (for recombining sequence), that is frequently recombined in mouse lambda light chain-producing B lymphocytes. Using Southern blot analyses of myelomas and mouse-Chinese hamster fusion cell lines, we found that RS DNA sequences are located on mouse chromosome 6, evidently more than 15 kilobases downstream of the kappa light-chain locus. We find that a typical recognition site for Ig gene recombination is situated within germ-line RS sequences near the recombination points observed in at least two lambda chain-producing cell lines. This represents a complete and functional Ig recognition site that is not directly associated with Ig genes. We also characterized a recombined RS segment isolated from the cell line BM18-4.13.9. This recombined segment has a variable region kappa light chain gene (V kappa) joined directly to RS sequences. Our results suggest that the deletion of the kappa light chain constant region (C kappa) exon in many lambda chain-producing B cells is the result of RS recombination and that C kappa deletion may be mediated by the same processes as antibody gene V-J joining (J = joining segment gene). We discuss the potential biological significance of RS DNA recombination in B-cell maturation.

PubMed Disclaimer

Cited by

Diversity of the mouse T cell receptor C gamma 1 gene: structural analysis in C57BL/Ka.
Rathbun GA, Born W, Kuziel WA, Tucker PW. Rathbun GA, et al. Immunogenetics. 1988;27(2):121-6. doi: 10.1007/BF00351085. Immunogenetics. 1988. PMID: 2891611
Genomic organization and evolution of immunoglobulin kappa gene enhancers and kappa deleting element in mammals.
Das S, Nikolaidis N, Nei M. Das S, et al. Mol Immunol. 2009 Sep;46(15):3171-7. doi: 10.1016/j.molimm.2009.05.180. Epub 2009 Jun 26. Mol Immunol. 2009. PMID: 19560204 Free PMC article.
B cell development in mice that lack one or both immunoglobulin kappa light chain genes.
Chen J, Trounstine M, Kurahara C, Young F, Kuo CC, Xu Y, Loring JF, Alt FW, Huszar D. Chen J, et al. EMBO J. 1993 Mar;12(3):821-30. doi: 10.1002/j.1460-2075.1993.tb05722.x. EMBO J. 1993. PMID: 8458340 Free PMC article.
Alternative mechanisms of receptor editing in autoreactive B cells.
Kalinina O, Doyle-Cooper CM, Miksanek J, Meng W, Prak EL, Weigert MG. Kalinina O, et al. Proc Natl Acad Sci U S A. 2011 Apr 26;108(17):7125-30. doi: 10.1073/pnas.1019389108. Epub 2011 Apr 6. Proc Natl Acad Sci U S A. 2011. PMID: 21471456 Free PMC article.
B-cell tolerance defects in the B6.Aec1/2 mouse model of Sjögren's syndrome.
Meng W, Li Y, Xue E, Satoh M, Peck AB, Cohen PL, Eisenberg RA, Luning Prak ET. Meng W, et al. J Clin Immunol. 2012 Jun;32(3):551-64. doi: 10.1007/s10875-012-9663-6. Epub 2012 Feb 17. J Clin Immunol. 2012. PMID: 22350147 Free PMC article.

References

1. J Mol Biol. 1975 Nov 5;98(3):503-17 - PubMed
1. Nature. 1983 Apr 14;302(5909):575-81 - PubMed
1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
1. Nature. 1979 Jul 26;280(5720):288-94 - PubMed
1. Proc Natl Acad Sci U S A. 1979 Jul;76(7):3450-4 - PubMed

Deletions of kappa chain constant region genes in mouse lambda chain-producing B cells involve intrachromosomal DNA recombinations similar to V-J joining - PubMed (original) (raw)