Viability of Escherichia coli K-12 DNA adenine methylase (dam) mutants requires increased expression of specific genes in the SOS regulon - PubMed (original) (raw)

Viability of Escherichia coli K-12 DNA adenine methylase (dam) mutants requires increased expression of specific genes in the SOS regulon

K R Peterson et al. Mol Gen Genet. 1985.

Abstract

We have examined the level of expression of the SOS regulon in cells lacking DNA adenine methylase activity (dam-). Mud (Ap, lac) fusions to several SOS operons (recA, lexA, uvrA, uvrB, uvrD, sulA, dinD and dinF) were found to express higher levels of beta-galactosidase in dam- strains than in isogenic dam+ strains. The attempted construction of dam- strains that were also mutant in one of several SOS genes indicated that the viability of methylase-deficient strains correlates with the inactivation of the SOS repressor (LexA protein). Consistent with this, the wild-type functions of two LexA-repressed genes (recA and ruv) appear to be required for dam- strain viability.

PubMed Disclaimer

References

    1. J Bacteriol. 1984 Jan;157(1):190-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1985 May;82(10):3325-9 - PubMed
    1. Mol Gen Genet. 1975 Dec 1;141(3):189-205 - PubMed
    1. Bacteriol Rev. 1976 Dec;40(4):869-907 - PubMed
    1. J Bacteriol. 1983 Nov;156(2):970-4 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources