Activators of protein kinase C down-regulate and phosphorylate the T3/T-cell antigen receptor complex of human T lymphocytes - PubMed (original) (raw)

Activators of protein kinase C down-regulate and phosphorylate the T3/T-cell antigen receptor complex of human T lymphocytes

D A Cantrell et al. Proc Natl Acad Sci U S A. 1985 Dec.

Abstract

As judged by indirect immunofluorescence, phorbol 12,13-dibutyrate and 1-oleoyl-2-acetylglycerol induced a rapid, concentration-dependent decrease of about 50% in the surface expression of the T3 antigen on human T lymphoblasts, and of T3 and the T-cell antigen receptor on HPB-ALL cells. Direct binding experiments using 125I-labeled antibody indicated that the reduction in T3 expression corresponded to a decrease in the number of antigen molecules rather than a change in their affinity. Biochemical analyses revealed that phorbol dibutyrate induced a rapid, prominent phosphorylation of the T3 Mr 26,000 gamma chain and to a lesser extent of the Mr 21,000 delta chain. No phosphorylation of the T3 epsilon chain or of the alpha and beta subunits of the T-cell antigen receptor was detected. The data suggest that protein kinase C induces a phosphorylation of the T3 gamma and delta chains that may lead to the down-regulation of the T3/T-cell antigen receptor complex.

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References

1. 1. Cell. 1983 Oct;34(3):717-26 - PubMed
2. 1. Eur J Immunol. 1984 Mar;14(3):273-5 - PubMed
3. 1. J Immunol. 1980 Jun;124(6):2708-13 - PubMed
4. 1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
5. 1. Cell. 1984 Apr;36(4):897-906 - PubMed

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