Influence of allelic polymorphism on the assembly and surface expression of class II MHC (Ia) molecules - PubMed (original) (raw)
Influence of allelic polymorphism on the assembly and surface expression of class II MHC (Ia) molecules
R N Germain et al. Cell. 1985 Nov.
Abstract
Cell surface Ia expression was examined following transfection of murine alpha and beta class II major histocompatibility complex (MHC) genes into L cells. Although haplotype-matched (e.g., Ak beta Ak alpha) gene pairs yielded high expression in primary transfectants, haplotype-mismatched pairs (e.g., Ak beta Ad alpha) gave unexpectedly low expression. RNA analysis revealed a requirement for greater A beta, and particularly A alpha transcript levels in haplotype-mismatched vs. haplotype-matched transfectants with similar levels of membrane Ia. A beta allelic control of this assembly/expression process was mapped to the NH2-terminal (beta 1) domain, the locus of high intraspecies polymorphism. These data on the effects of allelic variation on Ia levels provide a possible explanation for the strong linkage disequilibrium of A alpha and A beta genes and may account for the current molecular organization of the I region of the MHC.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials