Altered retinal metabolism in diabetes. I. Microanalysis of lipid, glucose, sorbitol, and myo-inositol in the choroid and in the individual layers of the rabbit retina - PubMed (original) (raw)
. 1986 Mar 25;261(9):4046-51.
- PMID: 3949802
Free article
Altered retinal metabolism in diabetes. I. Microanalysis of lipid, glucose, sorbitol, and myo-inositol in the choroid and in the individual layers of the rabbit retina
L C MacGregor et al. J Biol Chem. 1986.
Free article
Abstract
Total lipid, sucrose, glucose, sorbitol and myo-inositol contents in individual layers from normal and alloxan-diabetic rabbit retinas were measured using gravimetric and enzymatic microtechniques. Pure samples of nine retinal layers were microdissected from freeze-dried retinal cryosections. The lipid content was measured by loss of weight after ethanol and hexane extraction. Retinal lipid varied 3-fold across the retina and was not influenced by diabetes. Sucrose, glucose, sorbitol, and myo-inositol were measured with fluorimetric microassays. Sucrose infused intravenously prior to sampling the tissue did not traverse the outer blood retinal barrier of the normal or the diabetic retina. In both normals and diabetics, glucose followed a diffusional curve, with outer and inner retinal concentrations, respectively, equal to choroidal blood and vitreal glucose concentrations. Sorbitol was elevated in all retinal layers of diabetic animals. The peak sorbitol concentrations, of 2 mmol/kg defatted dry weight in diabetics, were not high enough to be osmotically significant. Retinal myo-inositol, of the order found in brain tissue (10-30 mmol/kg defatted dry tissue), was decreased by 22-40% in all retinal layers of the diabetics. The results indicate that diabetes affects the metabolism of retinal structures independently of the small blood vessel disease that is the hallmark of diabetes of long duration. It is conceivable that primary intraretinal metabolic alterations cause, aggravate, or perpetuate the well known degenerative processes that occur in retinal blood vessels in diabetes.
Similar articles
- Diabetes and the myo-inositol paradox.
Loy A, Lurie KG, Ghosh A, Wilson JM, MacGregor LC, Matschinsky FM. Loy A, et al. Diabetes. 1990 Oct;39(10):1305-12. doi: 10.2337/diab.39.10.1305. Diabetes. 1990. PMID: 2210080 - Experimental diabetes mellitus impairs the function of the retinal pigmented epithelium.
MacGregor LC, Matschinsky FM. MacGregor LC, et al. Metabolism. 1986 Apr;35(4 Suppl 1):28-34. doi: 10.1016/0026-0495(86)90184-8. Metabolism. 1986. PMID: 3007925 - Oxygen distribution and consumption within the retina in vascularised and avascular retinas and in animal models of retinal disease.
Yu DY, Cringle SJ. Yu DY, et al. Prog Retin Eye Res. 2001 Mar;20(2):175-208. doi: 10.1016/s1350-9462(00)00027-6. Prog Retin Eye Res. 2001. PMID: 11173251 Review. - Sorbitol, myo-inositol and sodium-potassium ATPase in diabetic peripheral nerve.
Greene DA. Greene DA. Drugs. 1986;32 Suppl 2:6-14. doi: 10.2165/00003495-198600322-00004. Drugs. 1986. PMID: 3024950 Review.
Cited by
- Metabolic Deficits in the Retina of a Familial Dysautonomia Mouse Model.
Costello SM, Schultz A, Smith D, Horan D, Chaverra M, Tripet B, George L, Bothner B, Lefcort F, Copié V. Costello SM, et al. Metabolites. 2024 Jul 31;14(8):423. doi: 10.3390/metabo14080423. Metabolites. 2024. PMID: 39195519 Free PMC article. - The Role of Aldose Reductase in Polyol Pathway: An Emerging Pharmacological Target in Diabetic Complications and Associated Morbidities.
Gupta JK. Gupta JK. Curr Pharm Biotechnol. 2024;25(9):1073-1081. doi: 10.2174/1389201025666230830125147. Curr Pharm Biotechnol. 2024. PMID: 37649296 Review. - The Protective Effects of Neurotrophins and MicroRNA in Diabetic Retinopathy, Nephropathy and Heart Failure via Regulating Endothelial Function.
Shityakov S, Nagai M, Ergün S, Braunger BM, Förster CY. Shityakov S, et al. Biomolecules. 2022 Aug 12;12(8):1113. doi: 10.3390/biom12081113. Biomolecules. 2022. PMID: 36009007 Free PMC article. Review. - Alpha-Smooth Muscle Actin Expression and Parafoveal Blood Flow Pathways Are Altered in Preclinical Diabetic Retinopathy.
An D, Chung-Wah-Cheong J, Yu DY, Balaratnasingam C. An D, et al. Invest Ophthalmol Vis Sci. 2022 May 2;63(5):8. doi: 10.1167/iovs.63.5.8. Invest Ophthalmol Vis Sci. 2022. PMID: 35522303 Free PMC article. - Comparison of choroidal thickness in eyes of diabetic patients with eyes of healthy individuals using optical coherence tomography in a tertiary care hospital.
Hassan H, Cheema A, Tahir MA, Nawaz HN. Hassan H, et al. Pak J Med Sci. 2022 Jan-Feb;38(1):254-260. doi: 10.12669/pjms.38.1.4443. Pak J Med Sci. 2022. PMID: 35035435 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources