A modified method to induce immune polyclonal ascites fluid in BALB/c mice using Sp2/0-Ag14 cells - PubMed (original) (raw)
A modified method to induce immune polyclonal ascites fluid in BALB/c mice using Sp2/0-Ag14 cells
M J Lacy et al. J Immunol Methods. 1986.
Abstract
Immunized BALB/c mice were injected with 10(6) viable Sp2/0-Ag14 cells to induce ascites fluid concurrently with maximum serum antibody activity. Ascites fluids developed in a systematic manner, yielded large amounts of specific polyclonal antibody even when the mice were injected with relatively small amounts of immunogen. A combination of an initial pristane injection prior to 3 intraperitoneal immunizations of immunogen emulsified in Freund's complete adjuvant (total volume of 0.1-0.2 ml per injection), followed 4 days later by injection of 10(6) viable Sp2/0-Ag14 cells, resulted in a consistent production of ascites fluid with substantial specific antibody activity.
Similar articles
- Monoclonal antibody production by hybridoma growth in Freund's adjuvant primed mice.
Mueller UW, Hawes CS, Jones WR. Mueller UW, et al. J Immunol Methods. 1986 Mar 13;87(2):193-6. doi: 10.1016/0022-1759(86)90530-2. J Immunol Methods. 1986. PMID: 3950429 - Production of polyclonal antibodies in ascitic fluid of mice: time and dose relationships.
Cartledge C, McLean C, Landon J. Cartledge C, et al. J Immunoassay. 1992;13(3):339-53. doi: 10.1080/15321819208021237. J Immunoassay. 1992. PMID: 1506456 - Production of polyclonal antibodies in ascitic fluid of mice: technique and applications.
Overkamp D, Mohammed-Ali S, Cartledge C, Landon J. Overkamp D, et al. J Immunoassay. 1988;9(1):51-68. doi: 10.1080/01971528808053210. J Immunoassay. 1988. PMID: 3283169 - Development of transplantable ascites tumours which continuously produce polyclonal antibodies in pristane primed BALB/c mice immunized with bacterial antigens and complete Freund's adjuvant.
Cevenini R, Sambri V, Pileri S, Ratti G, La Placa M. Cevenini R, et al. J Immunol Methods. 1991 Jun 24;140(1):111-8. doi: 10.1016/0022-1759(91)90132-y. J Immunol Methods. 1991. PMID: 2061607 - Alternatives to pristane priming for ascitic fluid and monoclonal antibody production.
Gillette RW. Gillette RW. J Immunol Methods. 1987 May 4;99(1):21-3. doi: 10.1016/0022-1759(87)90027-5. J Immunol Methods. 1987. PMID: 3571991
Cited by
- Ubiquitination of the bacterial inositol phosphatase, SopB, regulates its biological activity at the plasma membrane.
Knodler LA, Winfree S, Drecktrah D, Ireland R, Steele-Mortimer O. Knodler LA, et al. Cell Microbiol. 2009 Nov;11(11):1652-70. doi: 10.1111/j.1462-5822.2009.01356.x. Epub 2009 Jul 13. Cell Microbiol. 2009. PMID: 19614667 Free PMC article. - Interaction of mammalian Hsp22 with lipid membranes.
Chowdary TK, Raman B, Ramakrishna T, Rao ChM. Chowdary TK, et al. Biochem J. 2007 Jan 15;401(2):437-45. doi: 10.1042/BJ20061046. Biochem J. 2007. PMID: 17020537 Free PMC article. - Chlamydomonas reinhardtii Phosphoribulokinase : Sequence, Purification, and Kinetics.
Roesler KR, Ogren WL. Roesler KR, et al. Plant Physiol. 1990 May;93(1):188-93. doi: 10.1104/pp.93.1.188. Plant Physiol. 1990. PMID: 16667433 Free PMC article. - Coordinate Expression of Rubisco Activase and Rubisco during Barley Leaf Cell Development.
Zielinski RE, Werneke JM, Jenkins ME. Zielinski RE, et al. Plant Physiol. 1989 Jun;90(2):516-21. doi: 10.1104/pp.90.2.516. Plant Physiol. 1989. PMID: 16666801 Free PMC article. - Purification and species distribution of rubisco activase.
Salvucci ME, Werneke JM, Ogren WL, Portis AR. Salvucci ME, et al. Plant Physiol. 1987 Jul;84(3):930-6. doi: 10.1104/pp.84.3.930. Plant Physiol. 1987. PMID: 16665546 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical