Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP - PubMed (original) (raw)

Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP

S Mizusawa et al. Nucleic Acids Res. 1986.

Free PMC article

Abstract

The dideoxy chain termination method using deoxy-7-deazaguanosine triphosphate (dc7GTP) in place of dGTP was found to be very useful. Sequencing of a part of the human N-myc gene having 85% GC content is impossible by the original method using dGTP, because of compression of bands. However, the nucleotide sequence of this part was unambiguously determined by analysis of both strands by the modified method. Use of dc7GTP is concluded to improve the dideoxy chain termination method for DNA sequencing.

PubMed Disclaimer

Similar articles

• Interaction of deoxyguanosine nucleotide analogs with human telomerase.
  Tendian SW, Parker WB. Tendian SW, et al. Mol Pharmacol. 2000 Apr;57(4):695-9. doi: 10.1124/mol.57.4.695. Mol Pharmacol. 2000. PMID: 10727514
• Effective utilization of N2-ethyl-2'-deoxyguanosine triphosphate during DNA synthesis catalyzed by mammalian replicative DNA polymerases.
  Matsuda T, Terashima I, Matsumoto Y, Yabushita H, Matsui S, Shibutani S. Matsuda T, et al. Biochemistry. 1999 Jan 19;38(3):929-35. doi: 10.1021/bi982134j. Biochemistry. 1999. PMID: 9893988
• Improved cycle sequencing of GC-rich templates by a combination of nucleotide analogs.
  Motz M, Pääbo S, Kilger C. Motz M, et al. Biotechniques. 2000 Aug;29(2):268-70. doi: 10.2144/00292st01. Biotechniques. 2000. PMID: 10948427
• [Development of Damaged Nucleoside Mimics for Inhibition of Their Repair Enzymes].
  Taniguchi Y. Taniguchi Y. Yakugaku Zasshi. 2017;137(3):293-300. doi: 10.1248/yakushi.16-00231-2. Yakugaku Zasshi. 2017. PMID: 28250323 Review. Japanese.

Cited by

• Cloning and sequencing of the Escherichia coli chlEN operon involved in molybdopterin biosynthesis.
  Nohno T, Kasai Y, Saito T. Nohno T, et al. J Bacteriol. 1988 Sep;170(9):4097-102. doi: 10.1128/jb.170.9.4097-4102.1988. J Bacteriol. 1988. PMID: 3045084 Free PMC article.
• Nucleotide sequence of a cDNA clone encoding a precursor to stearoyl-(acyl-carrier-protein) desaturase from spinach, Spinacia oleracea.
  Nishida I, Beppu T, Matsuo T, Murata N. Nishida I, et al. Plant Mol Biol. 1992 Jul;19(4):711-3. doi: 10.1007/BF00026799. Plant Mol Biol. 1992. PMID: 1627785 No abstract available.
• Purification and characterization of endoglucanase C of Cellulomonas fimi, cloning of the gene, and analysis of in vivo transcripts of the gene.
  Moser B, Gilkes NR, Kilburn DG, Warren RA, Miller RC Jr. Moser B, et al. Appl Environ Microbiol. 1989 Oct;55(10):2480-7. doi: 10.1128/aem.55.10.2480-2487.1989. Appl Environ Microbiol. 1989. PMID: 2604391 Free PMC article.
• Spectrum of spontaneous mutations in a cDNA of the human hprt gene integrated in chromosomal DNA.
  Ikehata H, Akagi T, Kimura H, Akasaka S, Kato T. Ikehata H, et al. Mol Gen Genet. 1989 Nov;219(3):349-58. doi: 10.1007/BF00259606. Mol Gen Genet. 1989. PMID: 2622450

References

1. 1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
2. 1. Biochemistry. 1982 Aug 31;21(18):4338-43 - PubMed
3. 1. Nature. 1985 Jul 11-17;316(6024):160-2 - PubMed
4. 1. Gene. 1985;33(1):103-19 - PubMed
5. 1. J Mol Biol. 1983 May 5;166(1):1-19 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • PubMed Central
  • Silverchair Information Systems

• Other Literature Sources

  • The Lens - Patent Citations Database

• Miscellaneous

  • NCI CPTAC Assay Portal