A monoclonal anti-human platelet antibody: a new platelet aggregating substance - PubMed (original) (raw)
- PMID: 3967086
Free article
A monoclonal anti-human platelet antibody: a new platelet aggregating substance
M Higashihara et al. Blood. 1985 Feb.
Free article
Abstract
A monoclonal anti-human platelet antibody, TP82, is described, which caused irreversible aggregation of platelets in association with the release of adenosine triphosphate or [14C] serotonin, and which inhibited ristocetin-induced agglutination. Immunofluorescence assay showed that the antibody binds to platelets, megakaryocytes, and common acute lymphoblastic leukemia cells. The antibody (IgG1) immunoprecipitated a polypeptide of 23,000 daltons with an isoelectric point of about 7.0. The aggregation induced by the purified antibody and/or F(ab')2 fragments occurred in platelet-rich plasma and with washed platelets, but not with formalin-fixed washed platelets. TP82-induced aggregation was completely inhibited by disodium ethylendiaminotetraacetate, diltiazem, W-7, PGE1, and several metabolic inhibitors. At a concentration of apyrase or CP/CPK, which inhibited adenosine 5-diphosphate-induced aggregation. TP82-induced aggregation was only partially affected. Thrombin was not required for the antibody-mediated effects, since two thrombin inhibitors failed to block the reaction. The antibody, at least at a high concentration, induced platelet aggregation by a mechanism almost independent of thromboxane A2 formation, since cyclooxygenase inhibitors had little inhibitory effect on aggregation. TP82 monoclonal antibody is a new platelet-aggregating substance that interacts with a low-molecular-weight binding site on the platelet membrane.
Similar articles
- A monoclonal antibody directed against a granule membrane glycoprotein (GMP-140/PADGEM, P-selectin, CD62P) inhibits ristocetin-induced platelet aggregation.
Boukerche H, Ruchaud-Sparagano MH, Rouen C, Brochier J, Kaplan C, McGregor JL. Boukerche H, et al. Br J Haematol. 1996 Feb;92(2):442-51. doi: 10.1046/j.1365-2141.1996.d01-1485.x. Br J Haematol. 1996. PMID: 8603015 - Arachidonate metabolism, 5-hydroxytryptamine release and aggregation in human platelets activated by palmitaldehyde acetal phosphatidic acid.
Brammer JP, Maguire MH. Brammer JP, et al. Br J Pharmacol. 1984 May;82(1):61-72. doi: 10.1111/j.1476-5381.1984.tb16442.x. Br J Pharmacol. 1984. PMID: 6428500 Free PMC article. - Interrelation of platelet aggregation, release reaction and thromboxane A2 production.
Balduini CL, Bertolino G, Noris P, Sinigaglia F, Bisio A, Torti M. Balduini CL, et al. Biochem Biophys Res Commun. 1988 Oct 31;156(2):823-9. doi: 10.1016/s0006-291x(88)80918-5. Biochem Biophys Res Commun. 1988. PMID: 3190682
Cited by
- CD9, a tetraspanin target for cancer therapy?
Lorico A, Lorico-Rappa M, Karbanová J, Corbeil D, Pizzorno G. Lorico A, et al. Exp Biol Med (Maywood). 2021 May;246(9):1121-1138. doi: 10.1177/1535370220981855. Epub 2021 Feb 18. Exp Biol Med (Maywood). 2021. PMID: 33601913 Free PMC article. Review. - An antibody to the tetraspan membrane protein CD9 promotes neurite formation in a partially alpha3beta1 integrin-dependent manner.
Banerjee SA, Hadjiargyrou M, Patterson PH. Banerjee SA, et al. J Neurosci. 1997 Apr 15;17(8):2756-65. doi: 10.1523/JNEUROSCI.17-08-02756.1997. J Neurosci. 1997. PMID: 9092597 Free PMC article. - Mechanisms of platelet activation by a stimulatory antibody: cross-linking of a novel platelet receptor for monoclonal antibody F11 with the Fc gamma RII receptor.
Naik UP, Ehrlich YH, Kornecki E. Naik UP, et al. Biochem J. 1995 Aug 15;310 ( Pt 1)(Pt 1):155-62. doi: 10.1042/bj3100155. Biochem J. 1995. PMID: 7646439 Free PMC article. - Preparation of fibroblast-free cytotrophoblast cultures utilizing differential expression of the CD9 antigen.
Morrish DW, Shaw AR, Seehafer J, Bhardwaj D, Paras MT. Morrish DW, et al. In Vitro Cell Dev Biol. 1991 Apr;27A(4):303-6. doi: 10.1007/BF02630907. In Vitro Cell Dev Biol. 1991. PMID: 1856155 - Homotypic aggregation of murine T lymphocytes induced by anti-Thy-1 monoclonal antibodies.
Isobe K, Nakashima I. Isobe K, et al. Immunology. 1991 Jun;73(2):159-64. Immunology. 1991. PMID: 1676986 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous