Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface - PubMed (original) (raw)
Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface
G P Smith. Science. 1985.
Abstract
Foreign DNA fragments can be inserted into filamentous phage gene III to create a fusion protein with the foreign sequence in the middle. The fusion protein is incorporated into the virion, which retains infectivity and displays the foreign amino acids in immunologically accessible form. These "fusion phage" can be enriched more than 1000-fold over ordinary phage by affinity for antibody directed against the foreign sequence. Fusion phage may provide a simple way of cloning a gene when an antibody against the product of that gene is available.
Similar articles
- Antibody-selectable filamentous fd phage vectors: affinity purification of target genes.
Parmley SF, Smith GP. Parmley SF, et al. Gene. 1988 Dec 20;73(2):305-18. doi: 10.1016/0378-1119(88)90495-7. Gene. 1988. PMID: 3149606 - Filamentous fusion phage cloning vectors for the study of epitopes and design of vaccines.
Parmley SF, Smith GP. Parmley SF, et al. Adv Exp Med Biol. 1989;251:215-8. doi: 10.1007/978-1-4757-2046-4_21. Adv Exp Med Biol. 1989. PMID: 2481962 Review. - Phage antibodies: filamentous phage displaying antibody variable domains.
McCafferty J, Griffiths AD, Winter G, Chiswell DJ. McCafferty J, et al. Nature. 1990 Dec 6;348(6301):552-4. doi: 10.1038/348552a0. Nature. 1990. PMID: 2247164 - Structural constraints on the display of foreign peptides on filamentous bacteriophages.
Makowski L. Makowski L. Gene. 1993 Jun 15;128(1):5-11. doi: 10.1016/0378-1119(93)90146-t. Gene. 1993. PMID: 8508959 Review. - Expression of anti-Z-DNA single chain antibody variable fragment on the filamentous phage surface.
Maranhão AQ, Brígido MM. Maranhão AQ, et al. Braz J Med Biol Res. 2000 May;33(5):569-79. doi: 10.1590/s0100-879x2000000500012. Braz J Med Biol Res. 2000. PMID: 10775889
Cited by
- Stimuli-responsive self-regulating assembly of chiral colloids for robust size and shape control.
Wang S, Kang L, Salamon P, Wang X, Uchida N, Araoka F, Aida T, Dogic Z, Ishida Y. Wang S, et al. Nat Commun. 2024 Nov 15;15(1):9891. doi: 10.1038/s41467-024-54217-x. Nat Commun. 2024. PMID: 39543204 Free PMC article. - A New Bacterial Chassis for Enhanced Surface Display of Recombinant Proteins.
Zhang R, Ye N, Wang Z, Yang S, Li J. Zhang R, et al. Cell Mol Bioeng. 2024 Sep 13;17(5):453-465. doi: 10.1007/s12195-024-00819-w. eCollection 2024 Oct. Cell Mol Bioeng. 2024. PMID: 39513006 - Transitions in Immunoassay Leading to Next-Generation Lateral Flow Assays and Future Prospects.
Fujiuchi K, Aoki N, Ohtake T, Iwashita T, Kawasaki H. Fujiuchi K, et al. Biomedicines. 2024 Oct 6;12(10):2268. doi: 10.3390/biomedicines12102268. Biomedicines. 2024. PMID: 39457581 Free PMC article. Review. - Engineered M13-Derived Bacteriophages Capable of Gold Nanoparticle Synthesis and Nanogold Manipulations.
Karczewska-Golec J, Sadowska K, Golec P, Karczewski J, Węgrzyn G. Karczewska-Golec J, et al. Int J Mol Sci. 2024 Oct 18;25(20):11222. doi: 10.3390/ijms252011222. Int J Mol Sci. 2024. PMID: 39457002 Free PMC article. - Proximal Co-Translation Facilitates Detection of Weak Protein-Protein Interactions.
Kordonsky A, Gabay M, Rosinoff A, Avishid R, Flornetin A, Deouell N, Abd Alkhaleq T, Efron N, Milshtein S, Shifman JM, Gal M, Prag G. Kordonsky A, et al. Int J Mol Sci. 2024 Oct 16;25(20):11099. doi: 10.3390/ijms252011099. Int J Mol Sci. 2024. PMID: 39456880 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources