The adenovirus-2 early EIIa transcription unit possesses two overlapping promoters with different sequence requirements for EIa-dependent stimulation - PubMed (original) (raw)
The adenovirus-2 early EIIa transcription unit possesses two overlapping promoters with different sequence requirements for EIa-dependent stimulation
D A Zajchowski et al. EMBO J. 1985 May.
Abstract
The EIa-inducible, EIIa transcription unit of adenovirus-2 is transcribed early in infection from two start sites (+1 or EIIaE1 and -26 or EIIaE2), neither of which is preceded by canonical TATA box elements. Analysis of promoter deletion and linker scanning mutations for in vivo transcriptional activity after transfection into HeLa cells has indicated the existence of two overlapping promoters in the EIIaE gene. Two regions, each approximately 30 nucleotides upstream from start sites EIIaE1 and EIIaE2, function as TATA box substitutes. A sequence centered at position -42 (with respect to the major start site at position +1) is essential for transcription from both sites, while an element further upstream, localized between nucleotides -91 and -62, is also required for efficient EIIaE transcription, with the 3' border being dispensable for EIIaE2 transcription. Analysis of the entire series of EIIaE mutants, co-transfected with an EIa-containing plasmid, revealed that no unique sequence elements in the EIIaE1 promoter region between -97 and +1 were responsible for the stimulation of EIIaE1 transcription by EIa. In contrast, the EIa-mediated augmentation of EIIaE2 template activity was mainly dependent upon a sequence, the 5'-TTAAATTT-3' putative TATA box substitute, located around position -59.
Similar articles
- Sequence-specific trans-activation of the adenovirus EIIa early promoter by the viral EIV transcription unit.
Goding C, Jalinot P, Zajchowski D, Boeuf H, Kédinger C. Goding C, et al. EMBO J. 1985 Jun;4(6):1523-8. doi: 10.1002/j.1460-2075.1985.tb03812.x. EMBO J. 1985. PMID: 4029123 Free PMC article. - Adenovirus EIIA early promoter: transcriptional control elements and induction by the viral pre-early EIA gene, which appears to be sequence independent.
Murthy SC, Bhat GP, Thimmappaya B. Murthy SC, et al. Proc Natl Acad Sci U S A. 1985 Apr;82(8):2230-4. doi: 10.1073/pnas.82.8.2230. Proc Natl Acad Sci U S A. 1985. PMID: 3857577 Free PMC article. - Sequence-specific activation of transcription by adenovirus EIa products is observed in HeLa cells but not in 293 cells.
Leff T, Chambon P. Leff T, et al. Mol Cell Biol. 1986 Jan;6(1):201-8. doi: 10.1128/mcb.6.1.201-208.1986. Mol Cell Biol. 1986. PMID: 2946930 Free PMC article. - EIa-mediated stimulation of the adenovirus EIII promoter involves an enhancer element within the nearby EIIa promoter.
Zajchowski DA, Jalinot P, Kedinger C. Zajchowski DA, et al. J Virol. 1988 May;62(5):1762-7. doi: 10.1128/JVI.62.5.1762-1767.1988. J Virol. 1988. PMID: 2965764 Free PMC article. - Specific cellular proteins bind to critical promoter sequences of the adenovirus early EIIa promoter.
Boeuf H, Zajchowski DA, Tamura T, Hauss C, Kédinger C. Boeuf H, et al. Nucleic Acids Res. 1987 Jan 26;15(2):509-27. doi: 10.1093/nar/15.2.509. Nucleic Acids Res. 1987. PMID: 2950379 Free PMC article.
Cited by
- Unusual properties of adenovirus E2E transcription by RNA polymerase III.
Huang W, Flint SJ. Huang W, et al. J Virol. 2003 Apr;77(7):4015-24. doi: 10.1128/jvi.77.7.4015-4024.2003. J Virol. 2003. PMID: 12634361 Free PMC article. - Leukemia inhibitory factor-dependent transcriptional activation in embryonic stem cells.
Boeuf H, Hauss C, Graeve FD, Baran N, Kedinger C. Boeuf H, et al. J Cell Biol. 1997 Sep 22;138(6):1207-17. doi: 10.1083/jcb.138.6.1207. J Cell Biol. 1997. PMID: 9298977 Free PMC article. - Transcriptional activation by the adenovirus larger E1a product is mediated by members of the cellular transcription factor ATF family which can directly associate with E1a.
Chatton B, Bocco JL, Gaire M, Hauss C, Reimund B, Goetz J, Kedinger C. Chatton B, et al. Mol Cell Biol. 1993 Jan;13(1):561-70. doi: 10.1128/mcb.13.1.561-570.1993. Mol Cell Biol. 1993. PMID: 8417352 Free PMC article. - Activation of a dual adenovirus promoter containing nonconsensus TATA motifs in Schizosaccharomyces pombe: role of TATA sequences in the efficiency of transcription.
Swaminathan S, Malhotra P, Manohar CF, Dhar R, Thimmapaya B. Swaminathan S, et al. Nucleic Acids Res. 1993 Jun 11;21(11):2737-46. doi: 10.1093/nar/21.11.2737. Nucleic Acids Res. 1993. PMID: 8332470 Free PMC article. - The adenovirus E4-6/7 protein transactivates the E2 promoter by inducing dimerization of a heteromeric E2F complex.
Obert S, O'Connor RJ, Schmid S, Hearing P. Obert S, et al. Mol Cell Biol. 1994 Feb;14(2):1333-46. doi: 10.1128/mcb.14.2.1333-1346.1994. Mol Cell Biol. 1994. PMID: 8289811 Free PMC article.
References
- Nature. 1984 Apr 5-11;308(5959):513-9 - PubMed
- Nucleic Acids Res. 1985 Feb 25;13(4):1209-21 - PubMed
- Cell. 1984 May;37(1):273-83 - PubMed
- EMBO J. 1982;1(10):1279-85 - PubMed
- EMBO J. 1984 Apr;3(4):789-94 - PubMed
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases