Ribosomal protein production in normal and anucleolate Xenopus embryos: regulation at the posttranscriptional and translational levels - PubMed (original) (raw)
Comparative Study
Ribosomal protein production in normal and anucleolate Xenopus embryos: regulation at the posttranscriptional and translational levels
P Pierandrei-Amaldi et al. Cell. 1985 Aug.
Abstract
We have studied the regulation of ribosomal protein (r-protein) synthesis in Xenopus anucleolate mutants, which lack the genes for rRNA. The accumulation of mRNA for the two r-proteins analyzed parallels the controls up to stage 30. This mRNA is mobilized onto polysomes and is translated as in normal embryos, but r-proteins are unstable in the absence of rRNA to assemble with. A translational control of rp-mRNA distribution between polysomes and mRNPs is observed, but this is not due to an autogenous regulation by r-proteins. After stage 30 the amount of rp-mRNA declines specifically in the mutants because the transcripts are unstable. Considering the temporal correlation between this event and the onset of r-protein synthesis we suggest that an autogenous control operates at the level of transcript stability.
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