Identification of surface proteins on bovine leukocytes by a biotin-avidin protein blotting technique - PubMed (original) (raw)
Identification of surface proteins on bovine leukocytes by a biotin-avidin protein blotting technique
W L Hurley et al. J Immunol Methods. 1985.
Abstract
A method has been developed which covalently attaches biotin to proteins on the outer surface of leukocytes. These proteins are separated by polyacrylamide gel electrophoresis and transferred to nitrocellulose by protein blotting. Labeled proteins are detected using an avidin-peroxidase conjugate and color indicator. The method has been used to identify surface proteins specific to either polymorphonuclear leukocytes or mononuclear leukocytes prepared from bovine peripheral blood. The method provides a highly sensitive, non-radioactive means of examining alterations of surface proteins on leukocytes under differing functional and physiological conditions. Cell viability is not altered by this labeling method and labeled cells can be used to examine functions of surface proteins.
Similar articles
- Bovine leukocytes in mammary secretions during involution: surface protein changes.
Hurley WL, Finkelstein E. Hurley WL, et al. Am J Vet Res. 1986 Nov;47(11):2418-22. Am J Vet Res. 1986. PMID: 3789504 - Avidin- or streptavidin-biotin as a highly sensitive method to stain total protein on membranes.
Santora KE, Nelson SA, Lewis KA, LaRochelle WJ. Santora KE, et al. Mol Biotechnol. 2000 Jun;15(2):161-5. doi: 10.1385/MB:15:2:161. Mol Biotechnol. 2000. PMID: 10949830 - Visual detection of granulocyte surface antigens using the avidin-biotin complex.
Henke M, Yonemoto LM, Lazar GS, Gaidulis L, Hecht T, Santos S, Blume KG. Henke M, et al. J Histochem Cytochem. 1984 Jul;32(7):712-6. doi: 10.1177/32.7.6376617. J Histochem Cytochem. 1984. PMID: 6376617 - Biotinylation reagents for the study of cell surface proteins.
Elia G. Elia G. Proteomics. 2008 Oct;8(19):4012-24. doi: 10.1002/pmic.200800097. Proteomics. 2008. PMID: 18763706 Review.
Cited by
- Chemiluminescence detection of proteins from single cells.
Gillespie PG, Hudspeth AJ. Gillespie PG, et al. Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2563-7. doi: 10.1073/pnas.88.6.2563. Proc Natl Acad Sci U S A. 1991. PMID: 2006191 Free PMC article. - Polarized apical distribution of glycosyl-phosphatidylinositol-anchored proteins in a renal epithelial cell line.
Lisanti MP, Sargiacomo M, Graeve L, Saltiel AR, Rodriguez-Boulan E. Lisanti MP, et al. Proc Natl Acad Sci U S A. 1988 Dec;85(24):9557-61. doi: 10.1073/pnas.85.24.9557. Proc Natl Acad Sci U S A. 1988. PMID: 2974157 Free PMC article. - Biochemical and immunological characterization of major surface antigens of Sarcocystis muris and S. suicanis cyst merozoites.
Sommer I, Mehlhorn H, Rüger W. Sommer I, et al. Parasitol Res. 1991;77(3):204-11. doi: 10.1007/BF00930859. Parasitol Res. 1991. PMID: 1904582 - Identification of osteoblast-specific monoclonal antibodies.
Nakano T, Kimoto S, Tanikawa K, Kim KT, Higaki M, Kawase T, Saito S. Nakano T, et al. Calcif Tissue Int. 1989 Mar;44(3):220-7. doi: 10.1007/BF02556568. Calcif Tissue Int. 1989. PMID: 2493328 - Cells and cell lysates: a direct approach for engineering antibodies against membrane proteins using yeast surface display.
Tillotson BJ, Cho YK, Shusta EV. Tillotson BJ, et al. Methods. 2013 Mar 15;60(1):27-37. doi: 10.1016/j.ymeth.2012.03.010. Epub 2012 Mar 23. Methods. 2013. PMID: 22449570 Free PMC article. Review.