Established immunoglobulin producing myeloma (IgE) and lymphoblastoid (IgG) cell lines from an IgE myeloma patient - PubMed (original) (raw)
- PMID: 4097745
- PMCID: PMC1712861
Established immunoglobulin producing myeloma (IgE) and lymphoblastoid (IgG) cell lines from an IgE myeloma patient
K Nilsson et al. Clin Exp Immunol. 1970 Oct.
Abstract
Two IgEL and one IgGK producing cell lines have been established in vitro from peripheral blood and bone marrow of an E myeloma patient. Morphologic and immunologic studies indicate that cells of the IgE producing lines are derived from the same clone of myeloma cells which grew in vivo. They have remained essentially unaltered with a stable near-diploid karyotype and continuous production of intact IgE molecules during 18 months in culture. The IgGK producing line has all the characteristics ascribed to permanent lymphoblastoid lines and is considered to be of non-malignant lymphoid origin. The process of establishment is described and it is suggested that lymphoblastoid cells have a selective advantage over myeloma cells. Permanent myeloma cell lines can therefore probably be obtained only if cells capable of forming established lymphoblastoid lines are very few or absent in the original biopsy. The establishment of a permanent cell line continuously producing intact molecules of IgE should permit further studies on the biological role of this class of immunoglobulins.
Similar articles
- Immunoglobulin secretion by peripheral blood and bone marrow B cells in patients with multiple myeloma. Studies by the reverse haemolytic plaque assay.
Dammacco F, Vacca A, Altomare E, Campobasso N. Dammacco F, et al. Clin Exp Immunol. 1984 Sep;57(3):743-51. Clin Exp Immunol. 1984. PMID: 6380841 Free PMC article. - [Immunofluorescence optical studies for the differentiation of monoclonal and polyclonal increases in immunoglobulin producing bone marrow cells].
Morell A, Mosimann W, Skvaril F, Barandun S. Morell A, et al. Schweiz Med Wochenschr. 1974 Sep 28;104(39):1395-6. Schweiz Med Wochenschr. 1974. PMID: 4214489 German. No abstract available. - Use of tracer techniques in studies on immunoglobulin-producing cells.
Zucker-Franklin D. Zucker-Franklin D. J Histochem Cytochem. 1973 Mar;21(3):209-17. doi: 10.1177/21.3.209. J Histochem Cytochem. 1973. PMID: 4121416 Review. No abstract available. - [An autopsy case of IgE myeloma].
Chiyoda S, Morikawa T, Tsukasaki K, Nakajima Y. Chiyoda S, et al. Rinsho Ketsueki. 1989 May;30(5):686-90. Rinsho Ketsueki. 1989. PMID: 2507800 Review. Japanese.
Cited by
- TRAPnSeq allows high-throughput profiling of antigen-specific antibody-secreting cells.
Asrat S, Devlin JC, Vecchione A, Klotz B, Setliff I, Srivastava D, Limnander A, Rafique A, Adler C, Porter S, Murphy AJ, Atwal GS, Sleeman MA, Lim WK, Orengo JM. Asrat S, et al. Cell Rep Methods. 2023 Jul 7;3(7):100522. doi: 10.1016/j.crmeth.2023.100522. eCollection 2023 Jul 24. Cell Rep Methods. 2023. PMID: 37533642 Free PMC article. - Mutational analysis and protein profiling predict drug sensitivity in multiple myeloma cell lines.
Giliberto M, Santana LM, Holien T, Misund K, Nakken S, Vodak D, Hovig E, Meza-Zepeda LA, Coward E, Waage A, Taskén K, Skånland SS. Giliberto M, et al. Front Oncol. 2022 Nov 29;12:1040730. doi: 10.3389/fonc.2022.1040730. eCollection 2022. Front Oncol. 2022. PMID: 36523963 Free PMC article. - Standardization of Workflow and Flow Cytometry Panels for Quantitative Expression Profiling of Surface Antigens on Blood Leukocyte Subsets: An HCDM CDMaps Initiative.
Kužílková D, Puñet-Ortiz J, Aui PM, Fernández J, Fišer K, Engel P, van Zelm MC, Kalina T. Kužílková D, et al. Front Immunol. 2022 Feb 11;13:827898. doi: 10.3389/fimmu.2022.827898. eCollection 2022. Front Immunol. 2022. PMID: 35222411 Free PMC article. - Ex vivo drug sensitivity screening in multiple myeloma identifies drug combinations that act synergistically.
Giliberto M, Thimiri Govinda Raj DB, Cremaschi A, Skånland SS, Gade A, Tjønnfjord GE, Schjesvold F, Munthe LA, Taskén K. Giliberto M, et al. Mol Oncol. 2022 Mar;16(6):1241-1258. doi: 10.1002/1878-0261.13191. Epub 2022 Mar 12. Mol Oncol. 2022. PMID: 35148457 Free PMC article. - Tracing Human IgE B Cell Antigen Receptor-Bearing Cells With a Monoclonal Anti-Human IgE Antibody That Specifically Recognizes Non-Receptor-Bound IgE.
Zghaebi M, Byazrova M, Flicker S, Villazala-Merino S, Campion NJ, Stanek V, Tu A, Breiteneder H, Filatov A, Khaitov M, Niederberger-Leppin V, Eckl-Dorna J, Valenta R. Zghaebi M, et al. Front Immunol. 2021 Dec 20;12:803236. doi: 10.3389/fimmu.2021.803236. eCollection 2021. Front Immunol. 2021. PMID: 34987522 Free PMC article.
References
- Proc Soc Exp Biol Med. 1967 Aug-Sep;125(4):1246-50 - PubMed
- Immunology. 1967 Oct;13(4):381-94 - PubMed
- Immunology. 1968 Feb;14(2):265-72 - PubMed
- Int J Cancer. 1968 Mar 15;3(2):183-90 - PubMed
- Cancer. 1968 Aug;22(2):245-67 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials