Release and recovery of forespores from Bacillus cereus - PubMed (original) (raw)
Release and recovery of forespores from Bacillus cereus
A J Andreoli et al. J Bacteriol. 1973 Sep.
Abstract
A method is described which makes possible the release of immature forespores from sporulating cells at specific stages of development, from the completion of stage III through to mature spore formation. With the aid of zonal density gradient centrifugation, the method makes possible the recovery of quantities of forespores ample for biochemical and physical studies. With the capability to examine forespores and some mother cell components independently, we have established that several enzymes associated with the sporulation process are localized in the newly developed forespores. Studies showed that aspartate aminotransferase and alanine aminotransferase are associated with the forespores, whereas l-alanine dehydrogenase is found only in the mother cell cytoplasm.
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References
- J Biol Chem. 1951 Nov;193(1):265-75 - PubMed
- J Gen Microbiol. 1972 Aug;71(3):505-14 - PubMed
- Ann Intern Med. 1955 Aug;43(2):345-60 - PubMed
- Z Naturforsch B. 1958 Sep;13B(9):597-605 - PubMed
- J Bacteriol. 1963 Feb;85:451-60 - PubMed
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