Analysis of the attachment of replicating DNA to a nuclear matrix in mammalian interphase nuclei - PubMed (original) (raw)

Analysis of the attachment of replicating DNA to a nuclear matrix in mammalian interphase nuclei

P A Dijkwel et al. Nucleic Acids Res. 1979 Jan.

Free PMC article

Abstract

The attachment of replicating DNA to a rapidly sedimenting nuclear structure was investigated by digestion with various nucleases. When DNA was gradually removed by DNase I, pulse label incorporated during either 1 min or during 1 hour in the presence of arabinosylcytosine, remained preferentially attached to the nuclear structure. Single strand specific digestion by nuclease S1 or staphylococcal nuclease at low concentrations caused a release of about 30% of the pulse label, without significantly affecting the attachment of randomly labelled DNA. The released material had a low sedimentation coefficient and contained most of the Okasaki fragments. The remaining pulse label was less accessible to further digestion by double strand specific nuclease activity than the bulk DNA. The results suggest that an attachment of the replication fork to the nuclear structure occurs at sites behind but close to the branch point.

PubMed Disclaimer

Similar articles

• Analysis of the distribution of DNA repair patches in the DNA-nuclear matrix complex from human cells.
  Mullenders LH, Van Zeeland AA, Natarajan AT. Mullenders LH, et al. Biochim Biophys Acta. 1983 Sep 9;740(4):428-35. doi: 10.1016/0167-4781(83)90091-x. Biochim Biophys Acta. 1983. PMID: 6309237
• Organization of DNA replication in Physarum polycephalum. Attachment of origins of replicons and replication forks to the nuclear matrix.
  Aelen JM, Opstelten RJ, Wanka F. Aelen JM, et al. Nucleic Acids Res. 1983 Feb 25;11(4):1181-95. doi: 10.1093/nar/11.4.1181. Nucleic Acids Res. 1983. PMID: 6828380 Free PMC article.
• Attachment of origins of replication to the nuclear matrix and the chromosomal scaffold.
  van der Velden HM, van Willigen G, Wetzels RH, Wanka F. van der Velden HM, et al. FEBS Lett. 1984 Jun 4;171(1):13-6. doi: 10.1016/0014-5793(84)80451-2. FEBS Lett. 1984. PMID: 6723972
• Digestion of insect chromatin with micrococcal nuclease, DNase I and DNase I combined with single-strand specific nuclease S1.
  Schmidt ER. Schmidt ER. Nucleic Acids Res. 1977 Jul;4(7):2169-80. doi: 10.1093/nar/4.7.2169. Nucleic Acids Res. 1977. PMID: 909768 Free PMC article.
• Proteins tightly bound to HeLa cell DNA at nuclear matrix attachment sites.
  Bodnar JW, Jones CJ, Coombs DH, Pearson GD, Ward DC. Bodnar JW, et al. Mol Cell Biol. 1983 Sep;3(9):1567-79. doi: 10.1128/mcb.3.9.1567-1579.1983. Mol Cell Biol. 1983. PMID: 6355827 Free PMC article. Review.

Cited by

• Isolation and characterization of polyoma uncoating intermediates from the nuclei of infected mouse cells.
  Winston VD, Bolen JB, Consigli RA. Winston VD, et al. J Virol. 1980 Mar;33(3):1173-81. doi: 10.1128/JVI.33.3.1173-1181.1980. J Virol. 1980. PMID: 6245267 Free PMC article.
• Nuclear matrix and hnRNP share a common structural constituent associated with premessenger RNA.
  Gallinaro H, Puvion E, Kister L, Jacob M. Gallinaro H, et al. EMBO J. 1983;2(6):953-60. doi: 10.1002/j.1460-2075.1983.tb01527.x. EMBO J. 1983. PMID: 6557026 Free PMC article.
• Nuclear matrix associated DNA is preferentially repaired in normal human fibroblasts, exposed to a low dose of ultraviolet light but not in Cockayne's syndrome fibroblasts.
  Mullenders LH, van Kesteren van Leeuwen AC, van Zeeland AA, Natarajan AT. Mullenders LH, et al. Nucleic Acids Res. 1988 Nov 25;16(22):10607-22. doi: 10.1093/nar/16.22.10607. Nucleic Acids Res. 1988. PMID: 3205718 Free PMC article.
• Protein composition of the chromosomal scaffold and interphase nuclear matrix.
  Pieck AC, van der Velden HM, Rijken AA, Neis JM, Wanka F. Pieck AC, et al. Chromosoma. 1985;91(2):137-44. doi: 10.1007/BF00294058. Chromosoma. 1985. PMID: 3987439
• Nuclease resistance and the enrichment of native nuclear acceptor sites for the avian oviduct progesterone receptor.
  Hora J, Horton MJ, Toft DO, Spelsberg TC. Hora J, et al. Proc Natl Acad Sci U S A. 1986 Dec;83(23):8839-43. doi: 10.1073/pnas.83.23.8839. Proc Natl Acad Sci U S A. 1986. PMID: 3466161 Free PMC article.

References

1. 1. Proc Natl Acad Sci U S A. 1978 Jan;75(1):238-42 - PubMed
2. 1. Proc Natl Acad Sci U S A. 1978 Jan;75(1):233-7 - PubMed
3. 1. Proc Natl Acad Sci U S A. 1977 Jan;74(1):154-7 - PubMed
4. 1. Nucleic Acids Res. 1977 Jun;4(6):2083-97 - PubMed
5. 1. Biochim Biophys Acta. 1978 Oct 24;520(3):461-71 - PubMed

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • PubMed Central
  • Silverchair Information Systems