Purification and some kinetic properties of rat liver glucosamine synthetase - PubMed (original) (raw)
- PMID: 4255955
- PMCID: PMC1176648
- DOI: 10.1042/bj1210701
Purification and some kinetic properties of rat liver glucosamine synthetase
P J Winterburn et al. Biochem J. 1971 Feb.
Abstract
1. Glucosamine synthetase (l-glutamine-d-fructose 6-phosphate aminotransferase, EC 2.6.1.16) was purified about 300-fold from rat liver by two techniques. One procedure utilized the protective action of fructose 6-phosphate and gave a relatively stable preparation, the other yielded an unstable enzyme (half-life of about 20h), free of contaminant activities, on which kinetic experiments were performed. Although the properties of the two preparations showed slight differences, the unstabilized form could be converted into the stabilized form. 2. During preparation the enzyme retained its sensitivity to the feedback inhibitor, UDP-N-acetylglucosamine. 3. The reversibility of the enzyme-catalysed reaction could not be demonstrated. There was no apparent requirement for a cofactor. 4. The pH optimum was at 7.5, at which pH the reaction obeyed a Ping Pong Bi Bi rate equation. At pH values outside the range 6.9-7.6 and at temperatures below 29 degrees C the velocity was described by an ordered Bi Bi rate equation. 5. The molecular weight of the enzyme, determined by two procedures, was 360000-400000. 6. The aminotransferase was unable to utilize ammonia as a substrate.
Similar articles
- Effect of phosphoglucose isomerase and glucose 6-phosphate on UDP-N-acetylglucosamine inhibition of L-glutamine-D-fructose 6-phosphate aminotransferase.
Miyagi T, Tsuiki S. Miyagi T, et al. Biochim Biophys Acta. 1971 Oct;250(1):51-62. doi: 10.1016/0005-2744(71)90119-7. Biochim Biophys Acta. 1971. PMID: 5141677 No abstract available. - Glucosamine-6-phosphate synthase.
Zalkin H. Zalkin H. Methods Enzymol. 1985;113:278-81. doi: 10.1016/s0076-6879(85)13038-7. Methods Enzymol. 1985. PMID: 3003497 No abstract available. - Binding of substrates and modifiers to glucosamine synthetase.
Winterburn PJ, Phelps CF. Winterburn PJ, et al. Biochem J. 1971 Feb;121(4):721-30. doi: 10.1042/bj1210721. Biochem J. 1971. PMID: 5114980 Free PMC article. - Studies on the control of hexosamine biosynthesis by glucosamine synthetase.
Winterburn PJ, Phelps CF. Winterburn PJ, et al. Biochem J. 1971 Feb;121(4):711-20. doi: 10.1042/bj1210711. Biochem J. 1971. PMID: 5114979 Free PMC article. - Purification and characterization of cysteine conjugate transaminases from rat liver.
Tomisawa H, Ichimoto N, Takanohashi Y, Ichihara S, Fukazawa H, Tateishi M. Tomisawa H, et al. Xenobiotica. 1988 Sep;18(9):1015-28. doi: 10.3109/00498258809042224. Xenobiotica. 1988. PMID: 2852419
Cited by
- Transcriptional control of developmental processes by ecdysone inDrosophila virilis salivary glands.
Kress H. Kress H. Wilehm Roux Arch Dev Biol. 1977 Jun;182(2):107-116. doi: 10.1007/BF00848051. Wilehm Roux Arch Dev Biol. 1977. PMID: 28305265 - Regulation of the hexosamine biosynthetic pathway in the water mold Blastocladiella emersonii: Sensitivity to endproduct inhibition is dependent upon the life cycle phase.
Selitrennikoff CP, Dalley NE, Sonneborn DR. Selitrennikoff CP, et al. Proc Natl Acad Sci U S A. 1980 Oct;77(10):5998-6002. doi: 10.1073/pnas.77.10.5998. Proc Natl Acad Sci U S A. 1980. PMID: 16592895 Free PMC article. - UDP-sugar metabolism in Swarm rat chondrosarcoma chondrocytes.
Sweeney C, Mackintosh D, Mason RM. Sweeney C, et al. Biochem J. 1993 Mar 1;290 ( Pt 2)(Pt 2):563-70. doi: 10.1042/bj2900563. Biochem J. 1993. PMID: 8452547 Free PMC article. - Faecal diversion for Crohn's colitis: a model to study the role of the faecal stream in the inflammatory process.
Winslet MC, Allan A, Poxon V, Youngs D, Keighley MR. Winslet MC, et al. Gut. 1994 Feb;35(2):236-42. doi: 10.1136/gut.35.2.236. Gut. 1994. PMID: 8307475 Free PMC article.
References
- Biochem J. 1971 Feb;121(4):721-30 - PubMed
- Biochem J. 1965 Jun;95:868-75 - PubMed
- Arch Biochem Biophys. 1956 Nov;65(1):132-55 - PubMed
- J Biol Chem. 1967 Jul 10;242(13):3135-41 - PubMed
- Biochem J. 1964 May;91(2):222-33 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases