Generation of cytotoxic T lymphocytes in vitro. I. Response of normal and immune mouse spleen cells in mixed leukocyte cultures - PubMed (original) (raw)
Generation of cytotoxic T lymphocytes in vitro. I. Response of normal and immune mouse spleen cells in mixed leukocyte cultures
J C Cerottini et al. J Exp Med. 1974.
Abstract
Mouse cytotoxic T lymphocytes (CTL) were generated in mixed leukocyte cultures (MLC) using spleen cells as responding cells and irradiated allogeneic spleen cells as stimulating cells. Cytotoxicity was assessed by a quantitative (51)Cr assay system and the relative frequency of CTL in individual cell populations was estimated from dose-response curves. Inclusion of 2-mercaptoethanol in the MLC medium resulted in a 20-40-fold increase in the relative number of CTL generated at the peak of the response. Under these culture conditions, cell-mediated cytotoxic activity was detectable in MLC populations as early as 48 h after the onset of the cultures. When spleen cells from mice immunized with allogeneic tumor cells 2-4 mo previously were cultured with irradiated spleen cells of the same alloantigenic specificity (MLC-Imm), it was found that the cell-mediated cytotoxic response was detectable earlier and reached higher levels than that observed in a primary MLC. At the peak of the response, MLC-Imm populations were observed to lyse up to 50% of the target cells within 3 h at a lymphocyte: target cell ratio of 0.3:1. Immunological and physical characterization of the effector cells generated in MLC-Imm indicated that they were medium to large-sized T lymphocytes. Altogether, these studies suggested the existence of an anamnestic cell-mediated cytotoxic response in MLC-Imm.
Similar articles
- Generation of cytotoxic T lymphocytes in vitro. II. Effect of repeated exposure to alloantigens on the cytotoxic activity of long-term mixed leukocyte cultures.
Macdonald HR, Engers HD, Cerottini JC, Brunner KT. Macdonald HR, et al. J Exp Med. 1974 Sep 1;140(3):718-30. doi: 10.1084/jem.140.3.718. J Exp Med. 1974. PMID: 4278109 Free PMC article. - Generation of cytotoxic T lymphocytes in vitro. VI. Effect of cell density on response in mixed leukocyte cultures.
Fitch FW, Engers HD, MacDonald HR, Cerottini JC, Brunner KT. Fitch FW, et al. J Immunol. 1975 Dec;115(6):1688-94. J Immunol. 1975. PMID: 127012 - Generation of cytotoxic T lymphocytes in vitro. VII. Suppressive effect of irradiated MLC cells on CTL response.
Fitch FW, Engers HD, Cerottini JC, Bruner KT. Fitch FW, et al. J Immunol. 1976 Mar;116(3):716-23. J Immunol. 1976. PMID: 56399 - Generation of cytotoxic T lymphocytes in vitro. III. Velocity sedimentation studies of the differentiation and fate of effector cells in long-term mixed leukocyte cultures.
MacDonald HR, Cerottini JC, Brunner KT. MacDonald HR, et al. J Exp Med. 1974 Dec 1;140(6):1511-21. doi: 10.1084/jem.140.6.1511. J Exp Med. 1974. PMID: 4279270 Free PMC article. - Quantitative studies of the activation of cytotoxic lymphocyte precursor cells.
Miller RG, Teh HS, Harley E, Phillips RA. Miller RG, et al. Immunol Rev. 1977;35:38-58. doi: 10.1111/j.1600-065x.1977.tb00234.x. Immunol Rev. 1977. PMID: 330390 Review. No abstract available.
Cited by
- Role of Tumor-Associated Neutrophils in the Molecular Carcinogenesis of the Lung.
Taucher E, Taucher V, Fink-Neuboeck N, Lindenmann J, Smolle-Juettner FM. Taucher E, et al. Cancers (Basel). 2021 Nov 27;13(23):5972. doi: 10.3390/cancers13235972. Cancers (Basel). 2021. PMID: 34885082 Free PMC article. Review. - Potential alteration of COVID-19 by beta-mercaptoethanol.
Click RE. Click RE. Future Microbiol. 2020 Sep;15:1313-1318. doi: 10.2217/fmb-2020-0142. Epub 2020 Sep 21. Future Microbiol. 2020. PMID: 32951474 Free PMC article. No abstract available. - Neoantigen vaccine: an emerging tumor immunotherapy.
Peng M, Mo Y, Wang Y, Wu P, Zhang Y, Xiong F, Guo C, Wu X, Li Y, Li X, Li G, Xiong W, Zeng Z. Peng M, et al. Mol Cancer. 2019 Aug 23;18(1):128. doi: 10.1186/s12943-019-1055-6. Mol Cancer. 2019. PMID: 31443694 Free PMC article. Review. - Applications of Immunogenomics to Cancer.
Liu XS, Mardis ER. Liu XS, et al. Cell. 2017 Feb 9;168(4):600-612. doi: 10.1016/j.cell.2017.01.014. Cell. 2017. PMID: 28187283 Free PMC article. Review. - Tumor neoantigens: building a framework for personalized cancer immunotherapy.
Gubin MM, Artyomov MN, Mardis ER, Schreiber RD. Gubin MM, et al. J Clin Invest. 2015 Sep;125(9):3413-21. doi: 10.1172/JCI80008. Epub 2015 Aug 10. J Clin Invest. 2015. PMID: 26258412 Free PMC article. Review.
References
- Immunology. 1967 Oct;13(4):395-403 - PubMed
- Immunology. 1968 Feb;14(2):181-96 - PubMed
- J Cell Physiol. 1969 Jun;73(3):191-201 - PubMed
- J Immunol. 1965 Aug;95(2):230-45 - PubMed
- Science. 1970 Apr 3;168(3927):133-5 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources