Generation of cytotoxic T lymphocytes in vitro. II. Effect of repeated exposure to alloantigens on the cytotoxic activity of long-term mixed leukocyte cultures - PubMed (original) (raw)
Generation of cytotoxic T lymphocytes in vitro. II. Effect of repeated exposure to alloantigens on the cytotoxic activity of long-term mixed leukocyte cultures
H R Macdonald et al. J Exp Med. 1974.
Abstract
Mouse cytotoxic T lymphocytes (CTL) were generated in unidirectional mixed leukocyte cultures (MLC) using normal C57BL/6 spleen cells as responding cells and irradiated DBA/2 spleen cells as stimulating cells. Cytotoxicity was assayed on (51)Cr-labeled P-815 (DBA/2) target cells, and the relative frequency of CTL in individual cell populations was estimated from dose-response curves. Upon inclusion of 2-mercaptoethanol in the culture medium, it was found that significant CTL activity could be detected for as long as 3 wk in primary MLC. Reexposure of MLC cells to the original stimulating alloantigens after 14-41 days in culture resulted in significant cell proliferation and rapid regeneration of high levels of immunologically specific cytotoxicity. CTL activity in these secondary cultures increased dramatically within the first 24 h and reached higher peak levels than those found at the peak of the primary response. Furthermore, proliferation and reappearance of CTL activity could be demonstrated following each of as many as four sequential alloantigenic stimulations of the same initial cell population at 20-day intervals. Interestingly, cells recovered from MLC at the peak of the primary response on day 4 were insensitive to further allogeneic stimulation. Taken together, these results are consistent with the hypothesis that CTL differentiate in MLC to become long-lived memory cells which gradually lose their cytotoxic activity. Upon reexposure to specific alloantigen, such memory CTL rapidly regain their functional activity and proliferate to generate an expanded CTL population.
Similar articles
- Generation of cytotoxic T lymphocytes in vitro. I. Response of normal and immune mouse spleen cells in mixed leukocyte cultures.
Cerottini JC, Engers HD, Macdonald HR, Brunner T. Cerottini JC, et al. J Exp Med. 1974 Sep 1;140(3):703-17. doi: 10.1084/jem.140.3.703. J Exp Med. 1974. PMID: 4278108 Free PMC article. - Generation of cytotoxic T lymphocytes in vitro. VI. Effect of cell density on response in mixed leukocyte cultures.
Fitch FW, Engers HD, MacDonald HR, Cerottini JC, Brunner KT. Fitch FW, et al. J Immunol. 1975 Dec;115(6):1688-94. J Immunol. 1975. PMID: 127012 - Generation of cytotoxic T lymphocytes in vitro. VII. Suppressive effect of irradiated MLC cells on CTL response.
Fitch FW, Engers HD, Cerottini JC, Bruner KT. Fitch FW, et al. J Immunol. 1976 Mar;116(3):716-23. J Immunol. 1976. PMID: 56399 - Regulatory T cells in pregnancy. VI. Evidence for T-cell-mediated suppression of CTL generation toward paternal alloantigens.
Chaouat G, Monnot P, Hoffmann M, Voisin GA. Chaouat G, et al. Cell Immunol. 1982 Apr;68(2):322-31. doi: 10.1016/0008-8749(82)90116-2. Cell Immunol. 1982. PMID: 6212124 Review. No abstract available. - Clonal growth of T cells in vitro: preliminary attempts to a quantitative approach.
Watanabe T, Fathman CG, Coutinho A. Watanabe T, et al. Immunol Rev. 1977;35:3-37. doi: 10.1111/j.1600-065x.1977.tb00233.x. Immunol Rev. 1977. PMID: 70399 Review. No abstract available.
Cited by
- Progressive natural killer cell dysfunction associated with alterations in subset proportions and receptor expression in soft-tissue sarcoma patients.
Bücklein V, Adunka T, Mendler AN, Issels R, Subklewe M, Schmollinger JC, Noessner E. Bücklein V, et al. Oncoimmunology. 2016 Apr 29;5(7):e1178421. doi: 10.1080/2162402X.2016.1178421. eCollection 2016 Jul. Oncoimmunology. 2016. PMID: 27622032 Free PMC article. - Revisiting the first long-term culture of antigen-specific cytotoxic T cells.
Smith KA. Smith KA. Front Immunol. 2014 May 5;5:194. doi: 10.3389/fimmu.2014.00194. eCollection 2014. Front Immunol. 2014. PMID: 24829567 Free PMC article. Review. No abstract available. - Toward a molecular understanding of adaptive immunity: a chronology, part I.
Smith KA. Smith KA. Front Immunol. 2012 Dec 6;3:369. doi: 10.3389/fimmu.2012.00369. eCollection 2012. Front Immunol. 2012. PMID: 23230443 Free PMC article. - Targeting membrane heat-shock protein 70 (Hsp70) on tumors by cmHsp70.1 antibody.
Stangl S, Gehrmann M, Riegger J, Kuhs K, Riederer I, Sievert W, Hube K, Mocikat R, Dressel R, Kremmer E, Pockley AG, Friedrich L, Vigh L, Skerra A, Multhoff G. Stangl S, et al. Proc Natl Acad Sci U S A. 2011 Jan 11;108(2):733-8. doi: 10.1073/pnas.1016065108. Epub 2010 Dec 27. Proc Natl Acad Sci U S A. 2011. PMID: 21187371 Free PMC article. - Once a killer, always a killer: from cytotoxic T cell to memory cell.
Lefrançois L, Obar JJ. Lefrançois L, et al. Immunol Rev. 2010 May;235(1):206-18. doi: 10.1111/j.0105-2896.2010.00895.x. Immunol Rev. 2010. PMID: 20536565 Free PMC article. Review.
References
- Immunology. 1968 Feb;14(2):181-96 - PubMed
- Nature. 1970 Dec 26;228(5278):1308-9 - PubMed
- J Exp Med. 1971 Mar 1;133(3):442-53 - PubMed
- Cell Immunol. 1972 Feb;3(2):213-30 - PubMed
- Cell Immunol. 1972 May;4(1):39-50 - PubMed