An actin-like protein is a component of axonemes from Chlamydomonas flagella - PubMed (original) (raw)
Comparative Study
. 1979 Apr 10;254(7):2187-90.
- PMID: 429278
Free article
Comparative Study
An actin-like protein is a component of axonemes from Chlamydomonas flagella
G Piperno et al. J Biol Chem. 1979.
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Abstract
Several lines of evidence indicate that a component of axonemes from Chlamydomonas flagella is similar to actin from rabbit skeletal muscle. The polypeptide has an apparent molecular weight of 42,000 and in a pH gradient has the electrophoretic behavior of beta-actin. It was co-polymerized with rabbit actin and purified by affinity chromatography on DNase I-Sepharose. Incomplete proteolysis of mixed 35S-labeled axonemal protein and cold rabbit actin formed similar sets of peptides as analyzed by one-dimensional gel electrophoresis. The actin-like protein and the tubulin appear to be present in the axoneme in the molar ratio 1:60.
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