Isolation and partial characterization of a major inositol-containing lipid in baker's yeast, mannosyl-diinositol, diphosphoryl-ceramide - PubMed (original) (raw)

Isolation and partial characterization of a major inositol-containing lipid in baker's yeast, mannosyl-diinositol, diphosphoryl-ceramide

S Steiner et al. Proc Natl Acad Sci U S A. 1969 Nov.

Abstract

A polar lipid containing about 20 per cent of the lipid soluble inositol has been purified from cells of Saccharomyces cerevisiae. This compound labeled with (3)H-inositol, (32)P(i), or (14)C glucose was isolated from aqueous pyridine extracts by means of paper chromatography. Unlabeled compound was obtained by a differential solubility scheme. Analytical and degradation studies suggest that this compound can be formulated as a mannosyl-diinositol, diphosphoryl-ceramide. It can be converted by treatment with base or acid into a mannosylmonoinositol phosphoryl-ceramide, a composition which relates it to the basic structure of the phytoglycolipids.

PubMed Disclaimer

Similar articles

• Studies on the diversity of inositol-containing yeast phospholipids: incorporation of 2-deoxyglucose into lipid.
  Steiner S, Lester RL. Steiner S, et al. J Bacteriol. 1972 Jan;109(1):81-8. doi: 10.1128/jb.109.1.81-88.1972. J Bacteriol. 1972. PMID: 4333384 Free PMC article.
• The isolation and partial characterization of two novel sphingolipids from Neurospora crassa: di(inositolphosphoryl)ceramide and ((gal)3glu)ceramide.
  Lester RL, Smith SW, Wells GB, Rees DC, Angus WW. Lester RL, et al. J Biol Chem. 1974 Jun 10;249(11):3388-94. J Biol Chem. 1974. PMID: 4364652 No abstract available.
• Turnover of inositol and phosphorus containing lipids in Saccharomyces cerevisiae; extracellular accumulation of glycerophosphorylinositol derived from phosphatidylinositol.
  Angus WW, Lester RL. Angus WW, et al. Arch Biochem Biophys. 1972 Aug;151(2):483-95. doi: 10.1016/0003-9861(72)90525-5. Arch Biochem Biophys. 1972. PMID: 4339932 No abstract available.
• The determination of phytate and inositol phosphates.
  Oberleas D. Oberleas D. Methods Biochem Anal. 1971;20:87-101. doi: 10.1002/9780470110393.ch3. Methods Biochem Anal. 1971. PMID: 4336465 Review. No abstract available.
• Analytical studies using low levels of carbon-14 and tritium: a method for determining the labelling pattern in myo-inositol.
  Loewus FA. Loewus FA. Adv Tracer Methodol. 1965;2:163-7. doi: 10.1007/978-1-4684-8622-3_17. Adv Tracer Methodol. 1965. PMID: 5334291 Review. No abstract available.

Cited by

• Innovations in Antifungal Drug Discovery among Cell Envelope Synthesis Enzymes through Structural Insights.
  Zhou Y, Reynolds TB. Zhou Y, et al. J Fungi (Basel). 2024 Feb 22;10(3):171. doi: 10.3390/jof10030171. J Fungi (Basel). 2024. PMID: 38535180 Free PMC article. Review.
• Structural diversity and biological significance of glycosphingolipids in pathogenic and opportunistic fungi.
  Guimarães LL, Toledo MS, Ferreira FA, Straus AH, Takahashi HK. Guimarães LL, et al. Front Cell Infect Microbiol. 2014 Sep 25;4:138. doi: 10.3389/fcimb.2014.00138. eCollection 2014. Front Cell Infect Microbiol. 2014. PMID: 25309884 Free PMC article. Review.
• Phospholipid and sphingolipid metabolism in Leishmania.
  Zhang K, Beverley SM. Zhang K, et al. Mol Biochem Parasitol. 2010 Apr;170(2):55-64. doi: 10.1016/j.molbiopara.2009.12.004. Epub 2009 Dec 23. Mol Biochem Parasitol. 2010. PMID: 20026359 Free PMC article. Review.
• Degradation of host sphingomyelin is essential for Leishmania virulence.
  Zhang O, Wilson MC, Xu W, Hsu FF, Turk J, Kuhlmann FM, Wang Y, Soong L, Key P, Beverley SM, Zhang K. Zhang O, et al. PLoS Pathog. 2009 Dec;5(12):e1000692. doi: 10.1371/journal.ppat.1000692. Epub 2009 Dec 11. PLoS Pathog. 2009. PMID: 20011126 Free PMC article.

References

1. 1. J Biol Chem. 1968 Sep 25;243(18):4889-93 - PubMed
2. 1. J Bacteriol. 1969 Apr;98(1):10-5 - PubMed
3. 1. J Lipid Res. 1969 Jul;10(4):356-62 - PubMed
4. 1. J Biol Chem. 1953 Apr;201(2):825-38 - PubMed
5. 1. J Biol Chem. 1959 Mar;234(3):466-8 - PubMed

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Other Literature Sources

  • The Lens - Patent Citations