Ribonucleic acid polymerase activity in Sendai virions and nucleocapsid - PubMed (original) (raw)

Ribonucleic acid polymerase activity in Sendai virions and nucleocapsid

W S Robinson. J Virol. 1971 Jul.

Abstract

After dissociation of purified Sendai virus with the neutral detergent Nonidet P-40 and 2-mercaptoethanol, it catalyzed the incorporation of ribonucleoside triphosphates into an acid-insoluble product. The enzyme activity was associated with viral nucleocapsid as well as whole virions. The reaction product was ribonucleic acid (RNA) which annealed specifically with virion RNA. Sedimentation of the (3)H-RNA reaction product revealed two components, a 45S component with properties of double-stranded RNA and 4 to 6S component which appeared to be mostly single-stranded RNA.

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References

    1. J Mol Biol. 1966 Jun;18(1):195-203 - PubMed
    1. J Mol Biol. 1966 Jun;18(1):204-14 - PubMed
    1. Proc Natl Acad Sci U S A. 1967 Jul;58(1):134-41 - PubMed
    1. Proc Natl Acad Sci U S A. 1967 Dec;58(6):2280-7 - PubMed
    1. Virology. 1968 Aug;35(4):537-49 - PubMed

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