Degradation of DNA RNA hybrids by ribonuclease H and DNA polymerases of cellular and viral origin - PubMed (original) (raw)

Degradation of DNA RNA hybrids by ribonuclease H and DNA polymerases of cellular and viral origin

W Keller et al. Proc Natl Acad Sci U S A. 1972 Nov.

Abstract

Ribonuclease H from human KB cells, chick embryos, calf thymus, avian myeloblastosis virus, and Rous associated virus specifically degrades the RNA of DNA.RNA hybrids, producing mono- and oligoribonucleotides terminated in 5'-phosphates. The cellular RNase H is an endonuclease, whereas the viral enzyme appears to be an exonuclease. Viral DNA polymerase and RNase H copurify through all separation steps. Therefore, RNase H activity is an intrinsic part of the viral DNA polymerase. DNA.RNA hybrids are also degraded by nucleases associated with cellular DNA polymerases and by exonuclease III. However, these nucleases differ from RNase H in their ability to degrade both strands of DNA.RNA hybrids.

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References

    1. Science. 1969 Mar 28;163(3874):1410-8 - PubMed
    1. J Biol Chem. 1969 Jun 10;244(11):2996-3008 - PubMed
    1. J Biol Chem. 1969 Jun 25;244(12):3359-68 - PubMed
    1. Science. 1969 Oct 17;166(3903):393-5 - PubMed
    1. J Biol Chem. 1970 Jan 10;245(1):39-45 - PubMed

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