The interaction of soluble horseradish peroxidase with mouse peritoneal macrophages in vitro - PubMed (original) (raw)
The interaction of soluble horseradish peroxidase with mouse peritoneal macrophages in vitro
R M Steinman et al. J Cell Biol. 1972 Oct.
Abstract
The in vitro interaction of soluble horseradish peroxidase (HRP) with homogeneous mono layers of mouse macrophages has been studied using sensitive biochemical and cytochemical techniques. The compartmentalization of HRP in extracellular and intracellular sites has been quantitatively evaluated. A significant fraction is bound to a serum-derived layer, which coats the surface of culture vessels and may be removed by appropriate washes. Macrophages interiorize HRP as a solute in pinocytic vesicles without appreciable binding of the glycoprotein to the plasma membrane. Uptake is directly proportional to the concentration of HRP in the culture medium. 1 x 10(6) cells ingest 0.0025% of the administered load per hr over a wide range of concentrations. Cytochemically, all demonstrable HRP is sequestered within the endocytic vesicles and secondary lysosomes of the vacuolar apparatus. After uptake, the enzymatic activity of HRP is inactivated exponentially with a half-life of 7-9 hr, until enzyme is no longer detectable. When macrophages have pinocytosed trace-labeled HRP-(125)I, cell-associated isotope disappears with a t (1/2) of 20-30 hr and they release monoiodotyrosine-(125)I into the culture medium. We were unable to obtain evidence that significant amounts of HRP (>2%) can be exocytosed after uptake, can exist intact on the cell surface, or can be digested extracellularly. It is difficult to reconcile these observations with several of the postulated mechanisms whereby macrophages are thought to play a prominent role in the induction of an immune response.
Similar articles
- The interaction of particulate horseradish peroxidase (HRP)-anti HRP immune complexes with mouse peritoneal macrophages in vitro.
Steinman RM, Cohn ZA. Steinman RM, et al. J Cell Biol. 1972 Dec;55(3):616-34. doi: 10.1083/jcb.55.3.616. J Cell Biol. 1972. PMID: 4656704 Free PMC article. - Pinocytosis in fibroblasts. Quantitative studies in vitro.
Steinman RM, Silver JM, Cohn ZA. Steinman RM, et al. J Cell Biol. 1974 Dec;63(3):949-69. doi: 10.1083/jcb.63.3.949. J Cell Biol. 1974. PMID: 4140194 Free PMC article. - Phorbol esters and horseradish peroxidase stimulate pinocytosis and redirect the flow of pinocytosed fluid in macrophages.
Swanson JA, Yirinec BD, Silverstein SC. Swanson JA, et al. J Cell Biol. 1985 Mar;100(3):851-9. doi: 10.1083/jcb.100.3.851. J Cell Biol. 1985. PMID: 3972898 Free PMC article. - Membrane flow during pinocytosis. A stereologic analysis.
Steinman RM, Brodie SE, Cohn ZA. Steinman RM, et al. J Cell Biol. 1976 Mar;68(3):665-87. doi: 10.1083/jcb.68.3.665. J Cell Biol. 1976. PMID: 1030706 Free PMC article. - Endocytic uptake and vesicular transport of injected horseradish peroxidase in the vacuolar apparatus of rat liver cells.
Creemers J, Jacques PJ. Creemers J, et al. Exp Cell Res. 1971 Jul;67(1):188-203. doi: 10.1016/0014-4827(71)90634-3. Exp Cell Res. 1971. PMID: 4105560 No abstract available.
Cited by
- DCs at the center of help: Origins and evolution of the three-cell-type hypothesis.
Wu R, Murphy KM. Wu R, et al. J Exp Med. 2022 Jul 4;219(7):e20211519. doi: 10.1084/jem.20211519. Epub 2022 May 11. J Exp Med. 2022. PMID: 35543702 Free PMC article. - Regulation of Macropinocytosis by Diacylglycerol Kinase ζ.
Ard R, Mulatz K, Pomoransky JL, Parks RJ, Trinkle-Mulcahy L, Bell JC, Gee SH. Ard R, et al. PLoS One. 2015 Dec 23;10(12):e0144942. doi: 10.1371/journal.pone.0144942. eCollection 2015. PLoS One. 2015. PMID: 26701304 Free PMC article. - Polychlorinated Biphenyls (PCB 101, 153, and 180) Impair Murine Macrophage Responsiveness to Lipopolysaccharide: Involvement of NF-κB Pathway.
Santoro A, Ferrante MC, Di Guida F, Pirozzi C, Lama A, Simeoli R, Clausi MT, Monnolo A, Mollica MP, Mattace Raso G, Meli R. Santoro A, et al. Toxicol Sci. 2015 Sep;147(1):255-69. doi: 10.1093/toxsci/kfv127. Epub 2015 Jul 3. Toxicol Sci. 2015. PMID: 26141388 Free PMC article. - Determining the macropinocytic index of cells through a quantitative image-based assay.
Commisso C, Flinn RJ, Bar-Sagi D. Commisso C, et al. Nat Protoc. 2014 Jan;9(1):182-92. doi: 10.1038/nprot.2014.004. Epub 2014 Jan 2. Nat Protoc. 2014. PMID: 24385148 Free PMC article. - Fluorogenic probe for constitutive cellular endocytosis.
Levine MN, Hoang TT, Raines RT. Levine MN, et al. Chem Biol. 2013 Apr 18;20(4):614-8. doi: 10.1016/j.chembiol.2013.03.016. Chem Biol. 2013. PMID: 23601650 Free PMC article.
References
- J Exp Med. 1966 Apr 1;123(4):757-66 - PubMed
- J Exp Med. 1966 Oct 1;124(4):557-71 - PubMed
- J Exp Med. 1967 Feb 1;125(2):213-32 - PubMed
- J Histochem Cytochem. 1966 Apr;14(4):291-302 - PubMed
- J Chromatogr. 1966 Oct;24(2):465-8 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources