The effect of energization on the apparent Michaelis-Mentne constant for oxygen in mitochondrial respiration - PubMed (original) (raw)
The effect of energization on the apparent Michaelis-Mentne constant for oxygen in mitochondrial respiration
L C Petersen et al. Biochem J. 1974 Aug.
Abstract
Lineweaver-Burk plots of 1/v against 1/[O(2)] for rat liver mitochondrial respiration with succinate or ascorbate+NNN'N'-tetramethyl-p-phenylenediamine as substrates are non-linear. In state 3u (uncoupled by trifluoromethoxycarbonyl cyanide phenylhydrazone) such plots tend to be concave upward, whereas in state 4 (energized) the plots were concave downward. The apparent K(m) for oxygen is larger in state 4 than in state 3u, despite the higher turnover in the latter system. It is postulated that at least one reversible reaction occurs between cytochrome c and cytochrome c oxidase, whose rate is increased on energization (reversed electron transfer); a model including such a reaction is proposed which accounts semiquantitatively for the observations.
Similar articles
- The sum of flux control coefficients in the electron-transport chain of mitochondria.
Brand MD, Vallis BP, Kesseler A. Brand MD, et al. Eur J Biochem. 1994 Dec 15;226(3):819-29. doi: 10.1111/j.1432-1033.1994.00819.x. Eur J Biochem. 1994. PMID: 7813471 - Control of respiration in proteoliposomes containing cytochrome aa3. I. Stimulation by valinomycin and uncoupler.
Hansen FB, Miller M, Nicholls P. Hansen FB, et al. Biochim Biophys Acta. 1978 Jun 8;502(3):385-99. doi: 10.1016/0005-2728(78)90072-5. Biochim Biophys Acta. 1978. PMID: 207320 - The H+/e- stoicheiometry of respiration-linked proton translocation in the cytochrome system of mitochondria.
Papa S, Guerrieri F, Lorusso M, Izzo G, Boffoli D, Capuano F, Capitanio N, Altamura N. Papa S, et al. Biochem J. 1980 Oct 15;192(1):203-18. doi: 10.1042/bj1920203. Biochem J. 1980. PMID: 6272694 Free PMC article. - Kinetics of the cytochrome c oxidase and reductase reactions in energized and de-energized mitochondria.
Petersen LC, Degn H, Nicholls P. Petersen LC, et al. Can J Biochem. 1977 Jul;55(7):706-13. doi: 10.1139/o77-102. Can J Biochem. 1977. PMID: 196723 No abstract available. - Evolutionary adaptation of mitochondrial cytochrome c to its functional milieu.
Margoliash E. Margoliash E. UCLA Forum Med Sci. 1979;(21):299-321. doi: 10.1016/b978-0-12-643150-6.50024-5. UCLA Forum Med Sci. 1979. PMID: 233495 Review. No abstract available.
Cited by
- Searching for molecular hypoxia sensors among oxygen-dependent enzymes.
Li L, Shen S, Bickler P, Jacobson MP, Wu LF, Altschuler SJ. Li L, et al. Elife. 2023 Jul 26;12:e87705. doi: 10.7554/eLife.87705. Elife. 2023. PMID: 37494095 Free PMC article. Review. - Modeling the detailed kinetics of mitochondrial cytochrome c oxidase: Catalytic mechanism and nitric oxide inhibition.
Pannala VR, Camara AK, Dash RK. Pannala VR, et al. J Appl Physiol (1985). 2016 Nov 1;121(5):1196-1207. doi: 10.1152/japplphysiol.00524.2016. Epub 2016 Sep 15. J Appl Physiol (1985). 2016. PMID: 27633738 Free PMC article. - Maintenance of mitochondrial oxygen homeostasis by cosubstrate compensation.
Kueh HY, Niethammer P, Mitchison TJ. Kueh HY, et al. Biophys J. 2013 Mar 19;104(6):1338-48. doi: 10.1016/j.bpj.2013.01.030. Epub 2013 Mar 19. Biophys J. 2013. PMID: 23528093 Free PMC article. - A model of brain circulation and metabolism: NIRS signal changes during physiological challenges.
Banaji M, Mallet A, Elwell CE, Nicholls P, Cooper CE. Banaji M, et al. PLoS Comput Biol. 2008 Nov;4(11):e1000212. doi: 10.1371/journal.pcbi.1000212. Epub 2008 Nov 7. PLoS Comput Biol. 2008. PMID: 18989392 Free PMC article. - Functional implications of nitric oxide produced by mitochondria in mitochondrial metabolism.
Giulivi C. Giulivi C. Biochem J. 1998 Jun 15;332 ( Pt 3)(Pt 3):673-9. doi: 10.1042/bj3320673. Biochem J. 1998. PMID: 9620869 Free PMC article.
References
- Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1955;224(4):312-21 - PubMed
- Biochem J. 1957 Dec;67(4):558-72 - PubMed
- J Gen Physiol. 1965 Sep;49(1):Suppl:163-95 - PubMed
- Biochim Biophys Acta. 1973 Apr 5;292(3):534-53 - PubMed
- IEEE Trans Biomed Eng. 1973 Sep;20(5):384-7 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources