Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose - PubMed (original) (raw)

Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose

H Aviv et al. Proc Natl Acad Sci U S A. 1972 Jun.

Abstract

A convenient technique for the partial purification of large quantities of functional, poly(adenylic acid)-rich mRNA is described. The method depends upon annealing poly(adenylic acid)-rich mRNA to oligothymidylic acid-cellulose columns and its elution with buffers of low ionic strength. Biologically active rabbit globin mRNA has been purified by this procedure and assayed for its ability to direct the synthesis of rabbit globin in a cell-free extract of ascites tumor. Inasmuch as various mammalian mRNAs appear to be rich in poly(adenylic acid) and can likely be translated in the ascites cell-free extract, this approach should prove generally useful as an initial step in the isolation of specific mRNAs.

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References

1. 1. Biochemistry. 1968 Feb;7(2):668-74 - PubMed
2. 1. J Biol Chem. 1969 Mar 10;244(5):1314-24 - PubMed
3. 1. Biochemistry. 1969 Jul;8(7):3000-5 - PubMed
4. 1. Nature. 1970 Jan 10;225(5228):184-7 - PubMed
5. 1. Nature. 1970 Aug 15;227(5259):710-2 - PubMed

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