Rapid synthesis of oligodeoxyribonucleotides. III. Effect of added carboxylate ion on the efficiency of internucleotide bond formation. Solid-phase synthesis of the dodecanucleotide, d(pT-A-A-C-T-G-C-T-C-A-C-T) - PubMed (original) (raw)
Rapid synthesis of oligodeoxyribonucleotides. III. Effect of added carboxylate ion on the efficiency of internucleotide bond formation. Solid-phase synthesis of the dodecanucleotide, d(pT-A-A-C-T-G-C-T-C-A-C-T)
M J Gait et al. Nucleic Acids Res. 1979 Apr.
Free PMC article
Abstract
In solid-phase oligodeoxyribonucleotide synthesis the addition of benzoic acid to internucleotide phosphodiester bond-forming reactions lowers coupling yields by far greater amount than anticipated on a simple nucleotide-carboxylate molar ratio. High coupling yields were consistently obtained in synthesis of the dodecanucleotide, d(pT-A-A-C-T-G-C-T-C-A-C-T), using rigorously purified nucleotide starting materials.
Similar articles
- Rapid synthesis of oligodeoxyribonucleotides. II. Machine-aided solid-phase syntheses of two nonanucleotides and an octanucleotide.
Gait MJ, Sheppard RC. Gait MJ, et al. Nucleic Acids Res. 1977 Dec;4(12):4391-410. doi: 10.1093/nar/4.12.4391. Nucleic Acids Res. 1977. PMID: 600800 Free PMC article. - Solid phase synthesis of oligonucleotides on a crosslinked polyacrylmorpholide support.
Norris KE, Norris F, Brunfeldt K. Norris KE, et al. Nucleic Acids Symp Ser. 1980;(7):233-41. Nucleic Acids Symp Ser. 1980. PMID: 7255171 - Rapid synthesis of oligodeoxyribonucleotides: a new solid-phase method.
Gait MJ, Sheppard RC. Gait MJ, et al. Nucleic Acids Res. 1977 Apr;4(4):1135-58. doi: 10.1093/nar/4.4.1135. Nucleic Acids Res. 1977. PMID: 866189 Free PMC article. - Frontiers and approaches to chemical synthesis of oligodeoxyribonucleotides.
Abramova T. Abramova T. Molecules. 2013 Jan 15;18(1):1063-75. doi: 10.3390/molecules18011063. Molecules. 2013. PMID: 23322070 Free PMC article. Review.
Cited by
- A short primer for sequencing DNA cloned in the single-stranded phage vector M13mp2.
Anderson S, Gait MJ, Mayol L, Young IG. Anderson S, et al. Nucleic Acids Res. 1980 Apr 25;8(8):1731-43. doi: 10.1093/nar/8.8.1731. Nucleic Acids Res. 1980. PMID: 6253946 Free PMC article. - Rapid synthesis of oligodeoxyribonucleotides. VII. Solid phase synthesis of oligodeoxyribonucleotides by a continuous flow phosphotriester method on a kieselguhr-polyamide support.
Gait MJ, Matthes HW, Singh M, Sproat BS, Titmas RC. Gait MJ, et al. Nucleic Acids Res. 1982 Oct 25;10(20):6243-54. doi: 10.1093/nar/10.20.6243. Nucleic Acids Res. 1982. PMID: 7177846 Free PMC article. - Rapid synthesis of oligodeoxyribonucleotides. IV. Improved solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates.
Gait MJ, Singh M, Sheppard RC, Edge MD, Greene AR, Heathcliffe GR, Atkinson TC, Newton CR, Markham AF. Gait MJ, et al. Nucleic Acids Res. 1980 Mar 11;8(5):1081-96. doi: 10.1093/nar/8.5.1081. Nucleic Acids Res. 1980. PMID: 7443540 Free PMC article. - Complete sequence of an immunoglobulin mRNA using specific priming and the dideoxynucleotide method of RNA sequencing.
Hamlyn PH, Gait MJ, Milstein C. Hamlyn PH, et al. Nucleic Acids Res. 1981 Sep 25;9(18):4485-94. doi: 10.1093/nar/9.18.4485. Nucleic Acids Res. 1981. PMID: 6170937 Free PMC article. - Expression in Escherichia coli of chemically synthesized gene for the human immune interferon.
Tanaka S, Oshima T, Ohsuye K, Ono T, Mizono A, Ueno A, Nakazato H, Tsujimoto M, Higashi N, Noguchi T. Tanaka S, et al. Nucleic Acids Res. 1983 Mar 25;11(6):1707-23. doi: 10.1093/nar/11.6.1707. Nucleic Acids Res. 1983. PMID: 6300774 Free PMC article.
References
- Cell. 1978 Nov;15(3):1067-75 - PubMed
- Biochemistry. 1978 Apr 4;17(7):1257-67 - PubMed
- Nucleic Acids Res. 1977 Dec;4(12):4391-410 - PubMed
- Nucleic Acids Res. 1977 Apr;4(4):1135-58 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources