Methylated messenger RNA synthesis in vitro by purified reovirus - PubMed (original) (raw)

Methylated messenger RNA synthesis in vitro by purified reovirus

A J Shatkin. Proc Natl Acad Sci U S A. 1974 Aug.

Abstract

Purified human reovirus contains RNA methylase activity in addition to an RNA polymerase. Virions incubated under appropriate conditions in the presence of S-adenosyl-L-methionine synthesize mRNA that is specifically methylated in the 5'-terminal guanosine. Alkaline digestion of the methylated RNA released a 5'-terminal dinucleotide, ppG'pCp, indicating that the guanosine contains 2'-O-methylribose. The possible roles of methylation in viral and cellular mRNA function is discussed.

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