The 5'-terminal nucleotide sequences of the double-stranded RNA of human reovirus - PubMed (original) (raw)
The 5'-terminal nucleotide sequences of the double-stranded RNA of human reovirus
K Miura et al. Proc Natl Acad Sci U S A. 1974 Oct.
Abstract
The 5'-terminal nucleotide sequences of human reovirus double-stranded RNA were determined after labeling the RNA with [(32)P]phosphate by polynucleotide kinase. The 5' terminal were labeled to only a limited extent prior to sequential oxidation, beta-elimination, and phosphomonoesterase treatment, indicating that the terminal phosphates were in a modified, blocked configuration. Each genome segment, after removing the blocking group, contained the same two 5'-terminal sequences: GpApUp in one chain and G(*)pCp in the other. G(*)p is a derivative of guanylic acid, probably 2'-O-methyl-Gp, which renders the 5'-terminal sequence resistant to hydrolysis by alkali. The results indicate that the transcription of reovirus double-stranded RNA strats from the 3' end complementary to the G(*)pCp-terminal, resulting in the synthesis of single-stranded mRNA carrying the same 5' sequence as the G(*)pCp-chain. The presence of a modified nucleotide at the 5' terminus of the strand complementary to the mRNA template is a feature common to another double-stranded RNA virus, cytoplasmic polyhedrosis virus.
Similar articles
- Identification of the nucleotide sequences of the oligonucleotides present in reovirions.
Nichols JL, Bellamy AR, Joklik WK. Nichols JL, et al. Virology. 1972 Aug;49(2):562-72. doi: 10.1016/0042-6822(72)90507-7. Virology. 1972. PMID: 4340809 No abstract available. - 5'-Terminal nucleotide sequences of the double-stranded RNA of silkworm cytoplasmic polyhedrosis virus.
Miura K, Watanabe K, Sugiura M. Miura K, et al. J Mol Biol. 1974 Jun 15;86(1):31-48. doi: 10.1016/s0022-2836(74)80005-7. J Mol Biol. 1974. PMID: 4369455 No abstract available. - 5'-terminal nucleotide sequence of reovirus mRNA synthesized in vitro.
Nichols JL, Hay AJ, Joklik WK. Nichols JL, et al. Nat New Biol. 1972 Jan 26;235(56):105-7. doi: 10.1038/newbio235105a0. Nat New Biol. 1972. PMID: 4501191 No abstract available. - 5'-Terminal m-7G(5')ppp(5')G-m-p in vivo: identification in reovirus genome RNA.
Furuichi Y, Muthukrishnan S, Shatkin AJ. Furuichi Y, et al. Proc Natl Acad Sci U S A. 1975 Feb;72(2):742-5. doi: 10.1073/pnas.72.2.742. Proc Natl Acad Sci U S A. 1975. PMID: 1054852 Free PMC article. - Rapid nucleotide sequencing of DNA.
Friedmann T. Friedmann T. Am J Hum Genet. 1979 Jan;31(1):19-28. Am J Hum Genet. 1979. PMID: 373426 Free PMC article. Review. No abstract available.
Cited by
- Reovirus Efficiently Reassorts Genome Segments during Coinfection and Superinfection.
Thoner TW Jr, Meloy MM, Long JM, Diller JR, Slaughter JC, Ogden KM. Thoner TW Jr, et al. J Virol. 2022 Sep 28;96(18):e0091022. doi: 10.1128/jvi.00910-22. Epub 2022 Sep 12. J Virol. 2022. PMID: 36094315 Free PMC article. - Captivating Perplexities of Spinareovirinae 5' RNA Caps.
Kniert J, Lin QF, Shmulevitz M. Kniert J, et al. Viruses. 2021 Feb 13;13(2):294. doi: 10.3390/v13020294. Viruses. 2021. PMID: 33668598 Free PMC article. Review. - Recognition of Reovirus RNAs by the Innate Immune System.
Abad AT, Danthi P. Abad AT, et al. Viruses. 2020 Jun 20;12(6):667. doi: 10.3390/v12060667. Viruses. 2020. PMID: 32575691 Free PMC article. Review. - Non-coding RNAs and retroviruses.
Zhang X, Ma X, Jing S, Zhang H, Zhang Y. Zhang X, et al. Retrovirology. 2018 Feb 9;15(1):20. doi: 10.1186/s12977-018-0403-8. Retrovirology. 2018. PMID: 29426337 Free PMC article. Review. - Discovery of m(7)G-cap in eukaryotic mRNAs.
Furuichi Y. Furuichi Y. Proc Jpn Acad Ser B Phys Biol Sci. 2015;91(8):394-409. doi: 10.2183/pjab.91.394. Proc Jpn Acad Ser B Phys Biol Sci. 2015. PMID: 26460318 Free PMC article. Review.
References
- Seikagaku. 1967 Aug;39(8):455-62 - PubMed
- Nat New Biol. 1972 Jan 26;235(56):105-7 - PubMed
- Virology. 1973 May;53(1):283-6 - PubMed
- J Mol Biol. 1967 Jan 28;23(2):135-48 - PubMed
- J Biochem. 1973 Jul;74(1):117-25 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources