Conditional lethality of recA and recB derivatives of a strain of Escherichia coli K-12 with a temperature-sensitive deoxyribonucleic acid polymerase I - PubMed (original) (raw)
Conditional lethality of recA and recB derivatives of a strain of Escherichia coli K-12 with a temperature-sensitive deoxyribonucleic acid polymerase I
M Monk et al. J Bacteriol. 1972 Mar.
Abstract
We have isolated a strain of Escherichia coli K-12 carrying a mutation, polA12, that results in the synthesis of a temperature-sensitive deoxyribonucleic acid (DNA) polymerase I. The double mutants polA12 recA56 and polA12 recB21, constructed at 30 C, are inviable at 42 C. About 90% of the cells of both double mutants die after 2 hr of incubation at 42 C. Both double mutants filament at 42 C and show a dependence on high cell density for growth at 30 C. In polA12 recB21 cells at 42 C, DNA and protein synthesis gradually stop in parallel. In polA12 recA56 cells, DNA synthesis continues for at least 1 hr at 42 C, and there is extensive DNA degradation. The results suggest that the primary lesion in these double mutants is not in DNA replication per se.
Similar articles
- Genetic analysis of a temperature-resistant revertant of the conditional lethal Escherichia coli double mutant polA12 uvrE502.
Smirnov GB, Saenko AS. Smirnov GB, et al. J Bacteriol. 1974 Jul;119(1):1-8. doi: 10.1128/jb.119.1.1-8.1974. J Bacteriol. 1974. PMID: 4366022 Free PMC article. - Deoxyribonucleic acid synthesis in recA and recB derivatives of an Escherichia coli K-12 strain with a temperature-sensitive deoxyribonucleic acid polymerase I.
Monk M, Kinross J, Town C. Monk M, et al. J Bacteriol. 1973 Jun;114(3):1014-7. doi: 10.1128/jb.114.3.1014-1017.1973. J Bacteriol. 1973. PMID: 4576395 Free PMC article. - Isolation and characterization of an Escherichia coli K-12 mutant with a temperature-sensitive recA- phenotype.
Lloyd RG, Low B, Godson GN, Birge EA. Lloyd RG, et al. J Bacteriol. 1974 Oct;120(1):407-15. doi: 10.1128/jb.120.1.407-415.1974. J Bacteriol. 1974. PMID: 4607624 Free PMC article. - Excision repair characteristics of recB - res - and uvrC - strains of Escherichia coli.
Kato T. Kato T. J Bacteriol. 1972 Dec;112(3):1237-46. doi: 10.1128/jb.112.3.1237-1246.1972. J Bacteriol. 1972. PMID: 4344920 Free PMC article. - Pedigrees of some mutant strains of Escherichia coli K-12.
Bachmann BJ. Bachmann BJ. Bacteriol Rev. 1972 Dec;36(4):525-57. doi: 10.1128/br.36.4.525-557.1972. Bacteriol Rev. 1972. PMID: 4568763 Free PMC article. Review. No abstract available.
Cited by
- HslO ameliorates arrested ΔrecA polA cell growth and reduces DNA damage and oxidative stress responses.
Kaidow A, Ishii N, Suzuki S, Shiina T, Endoh K, Murakami Y, Kasahara H. Kaidow A, et al. Sci Rep. 2022 Dec 23;12(1):22182. doi: 10.1038/s41598-022-26703-z. Sci Rep. 2022. PMID: 36564489 Free PMC article. - Reactive oxygen species accumulation is synchronised with growth inhibition of temperature-sensitive recAts polA Escherichia coli.
Kaidow A, Ishii N, Suzuki S, Shiina T, Kasahara H. Kaidow A, et al. Arch Microbiol. 2022 Jun 16;204(7):396. doi: 10.1007/s00203-022-02957-z. Arch Microbiol. 2022. PMID: 35705748 Free PMC article. - Interaction with single-stranded DNA-binding protein localizes ribonuclease HI to DNA replication forks and facilitates R-loop removal.
Wolak C, Ma HJ, Soubry N, Sandler SJ, Reyes-Lamothe R, Keck JL. Wolak C, et al. Mol Microbiol. 2020 Sep;114(3):495-509. doi: 10.1111/mmi.14529. Epub 2020 Jun 4. Mol Microbiol. 2020. PMID: 32426857 Free PMC article. - IS_26_ Family Members IS_257_ and IS_1216_ Also Form Cointegrates by Copy-In and Targeted Conservative Routes.
Harmer CJ, Hall RM. Harmer CJ, et al. mSphere. 2020 Jan 8;5(1):e00811-19. doi: 10.1128/mSphere.00811-19. mSphere. 2020. PMID: 31915227 Free PMC article. - Temperature-sensitive DNA repair mutations in the cellular slime mould Dictyostelium discoideum.
Welker DL, Williams KL. Welker DL, et al. Curr Genet. 1981 Jul;3(3):167-71. doi: 10.1007/BF00429818. Curr Genet. 1981. PMID: 24190128
References
- J Mol Biol. 1971 Jun 14;58(2):631-4 - PubMed
- J Bacteriol. 1971 Apr;106(1):204-12 - PubMed
- Bacteriol Rev. 1970 Jun;34(2):155-75 - PubMed
- Proc Natl Acad Sci U S A. 1968 May;60(1):160-7 - PubMed
- Nature. 1971 Mar 5;230(5288):33-6 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Research Materials