Isolation and characterization of acid phosphatase mutants in Saccharomyces cerevisiae - PubMed (original) (raw)

Isolation and characterization of acid phosphatase mutants in Saccharomyces cerevisiae

A To-E et al. J Bacteriol. 1973 Feb.

Abstract

Saccharomyces cerevisiae strain H-42 seems to have two kinds of acid phosphatase: one which is constitutive and one which is repressible by inorganic phosphate. The constitutive enzyme was significantly unstable to heat inactivation, and its K(m) of 9.1 x 10(-4)m for p-nitrophenylphosphate was higher than that of the repressible enzyme (2.4 x 10(-4)m). The constitutive and the repressible acid phosphatases are specified by the phoC gene and by the phoB, phoD, or phoE gene, respectively. Results of tetrad analysis suggested that the phoC and phoE genes are linked to the lys2 locus on chromosome II. Since both repressible acid and alkaline phosphatases were affected simultaneously in the phoR, phoD, and phoS mutants, it was concluded that these enzymes were under the same regulatory mechanism or that they shared a common polypeptide. The phoR mutant produced acid phosphatase constitutively, and the phoR mutant allele was recessive to its wild-type counterpart. The phoS mutant showed a phenotype similar to that of a mutant defective in one of the phoB, phoD, or phoE genes. However, the results of genetic analysis of the phoS mutant clearly indicated that the phoS gene is not a structural gene for either of the repressible acid and alkaline phosphatases, but is a kind of regulatory gene. According to the proposed model, the phoS gene controls the expression of the phoR gene, and inorganic phosphate would act primarily as an inducer for the formation of the phoR product which represses phosphatase synthesis.

PubMed Disclaimer

Similar articles

• Isolation and characterization of recessive, constitutive mutations for repressible acid phosphatase synthesis in Saccharomyces cerevisiae.
  Ueda Y, To-E A, Oshima Y. Ueda Y, et al. J Bacteriol. 1975 Jun;122(3):911-22. doi: 10.1128/jb.122.3.911-922.1975. J Bacteriol. 1975. PMID: 1097406 Free PMC article.
• Genes coding for the structure of the acid phosphatases in Saccharomyces cerevisiae.
  Toh-e A, Kakimoto S. Toh-e A, et al. Mol Gen Genet. 1975 Dec 30;143(1):65-70. doi: 10.1007/BF00269421. Mol Gen Genet. 1975. PMID: 765744
• Characterization of a dominant, constitutive mutation, PHOO, for the repressible acid phosphatase synthesis in Saccharomyces cerevisiae.
  Toh-E A, Oshima Y. Toh-E A, et al. J Bacteriol. 1974 Nov;120(2):608-17. doi: 10.1128/jb.120.2.608-617.1974. J Bacteriol. 1974. PMID: 4616940 Free PMC article.
• Regulation of phosphatase synthesis in Saccharomyces cerevisiae--a review.
  Oshima Y, Ogawa N, Harashima S. Oshima Y, et al. Gene. 1996 Nov 7;179(1):171-7. doi: 10.1016/s0378-1119(96)00425-8. Gene. 1996. PMID: 8955644 Review.
• Isolation of regulatory mutants in Saccharomyces cerevisiae.
  Greer H, Fink GR. Greer H, et al. Methods Cell Biol. 1975;11:247-72. doi: 10.1016/s0091-679x(08)60327-1. Methods Cell Biol. 1975. PMID: 1102851 Review. No abstract available.

Cited by

• Regulation of the neutral phosphatase in Chlamydomonas reinhardi: an immunogenetic study of wild-type and mutant strains.
  Loppes R, Braipson J, Matagne RF, Sassen A, Ledoux L. Loppes R, et al. Biochem Genet. 1977 Dec;15(11-12):1147-57. doi: 10.1007/BF00484505. Biochem Genet. 1977. PMID: 203266
• Promoter occupancy is a major determinant of chromatin remodeling enzyme requirements.
  Dhasarathy A, Kladde MP. Dhasarathy A, et al. Mol Cell Biol. 2005 Apr;25(7):2698-707. doi: 10.1128/MCB.25.7.2698-2707.2005. Mol Cell Biol. 2005. PMID: 15767675 Free PMC article.
• Sequence and chromatin features guide DNA double-strand break resection initiation.
  Gnügge R, Reginato G, Cejka P, Symington LS. Gnügge R, et al. Mol Cell. 2023 Apr 20;83(8):1237-1250.e15. doi: 10.1016/j.molcel.2023.02.010. Epub 2023 Mar 13. Mol Cell. 2023. PMID: 36917982 Free PMC article.
• Genetic analysis of chromatin remodeling using budding yeast as a model.
  Steger DJ, O'Shea EK. Steger DJ, et al. Methods Enzymol. 2004;377:55-60. doi: 10.1016/S0076-6879(03)77002-5. Methods Enzymol. 2004. PMID: 14979017 Free PMC article.
• Acid phosphatase polypeptides in Saccharomyces cerevisiae are encoded by a differentially regulated multigene family.
  Rogers DT, Lemire JM, Bostian KA. Rogers DT, et al. Proc Natl Acad Sci U S A. 1982 Apr;79(7):2157-61. doi: 10.1073/pnas.79.7.2157. Proc Natl Acad Sci U S A. 1982. PMID: 6212932 Free PMC article.

References

1. 1. Genetica. 1956;28(1-2):165-76 - PubMed
2. 1. Biochemistry. 1963 Jan-Feb;2:126-31 - PubMed
3. 1. Biochim Biophys Acta. 1960 Mar 11;38:470-83 - PubMed
4. 1. J Bacteriol. 1962 Feb;83:297-300 - PubMed
5. 1. Genetics. 1968 Dec;60(4):735-42 - PubMed

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • BioCyc
  • Gene Ontology
  • Saccharomyces Genome Database