Temperature-sensitive mutants for the replication of plasmids in Escherichia coli. I. Isolation and specificity of host and plasmid mutations - PubMed (original) (raw)
Temperature-sensitive mutants for the replication of plasmids in Escherichia coli. I. Isolation and specificity of host and plasmid mutations
D T Kingsbury et al. Genetics. 1973 May.
Abstract
Temperature-sensitive mutants of Escherichia coli defective in the replication of the plasmid colicinogenic factor E1 (ColE(1)) were isolated following mutagenesis of E. coli K12 strain carrying the ColE(1) factor. Following the mutagenic treatment an enrichment procedure utilizing the replacement of thymine with bromouracil in the ColE(1) DNA duplicated at the restrictive temperature was used. The mutants isolated following this enrichment step were the result of a mutation event either in the host chromosome or in the ColE(1) plasmid. The host mutants fell into three phenotypic classes based on the effect each mutation had on the maintenance of a variety of other extrachromosomal DNA elements. Phenotypic class I mutations affected all E. coli plasmids, both the I and F sex factor types as well as the ColE(1) factor. Phenotypic class II mutations affected the maintenance of the ColE(1) and the F sex factor type plasmids and not the I type, while phenotypic class III mutations affected only ColE(1) replication. None of these mutations was found to have a significant effect on the replication of the E. coli chromosome. The plasmid-linked mutations fell into two phenotypic classes on the basis of the ability of the Flac episome to complement the mutation in the ColE(1) plasmid.
Similar articles
- Temperature-sensitive mutants for the replication of plasmids in Escherichia coli. II. Properties of host and plasmid mutations.
Kingsbury DT, Sieckmann DG, Helinski DR. Kingsbury DT, et al. Genetics. 1973 May;74(1):1-16. doi: 10.1093/genetics/74.1.1. Genetics. 1973. PMID: 4576230 Free PMC article. - Temperature-sensitive mutants for the replication of plasmids in Escherichia coli: requirement for deoxyribonucleic acid polymerase I in the replication of the plasmid ColE 1.
Kingsbury DT, Helinski DR. Kingsbury DT, et al. J Bacteriol. 1973 Jun;114(3):1116-24. doi: 10.1128/jb.114.3.1116-1124.1973. J Bacteriol. 1973. PMID: 4576398 Free PMC article. - Studies on plasmid replication. III. Isolation and characterization of replication-defective mutants.
Novick RP. Novick RP. Mol Gen Genet. 1974;135(2):131-47. doi: 10.1007/BF00264781. Mol Gen Genet. 1974. PMID: 4457754 - The genetics of transmissible plasmids.
Willetts N. Willetts N. Annu Rev Genet. 1972;6:257-68. doi: 10.1146/annurev.ge.06.120172.001353. Annu Rev Genet. 1972. PMID: 4352831 Review. No abstract available.
Cited by
- Plasmid ColE1 DNA replication in Escherichia coli strains temperature-sensitive for DNA replication.
Collins J, Williams P, Helinski DR. Collins J, et al. Mol Gen Genet. 1975;136(4):273-89. doi: 10.1007/BF00341713. Mol Gen Genet. 1975. PMID: 16094996 - Isolation and characterization of a composite plasmid Rms201 mutant temperature sensitive for replication.
Ike Y, Hashimoto H, Motohashi K, Fujisawa N, Mitsuhashi S. Ike Y, et al. J Bacteriol. 1980 Feb;141(2):577-83. doi: 10.1128/jb.141.2.577-583.1980. J Bacteriol. 1980. PMID: 6988387 Free PMC article. - Trimethoprim-induced DNA polymerase I deficiency in Escherichia coli K-12.
Mukhopadhyay P, Syrowik U, Palchaudhuri S. Mukhopadhyay P, et al. J Bacteriol. 1983 Jun;154(3):1098-103. doi: 10.1128/jb.154.3.1098-1103.1983. J Bacteriol. 1983. PMID: 6343343 Free PMC article. - Selection and characterization of ColE1 plasmid mutants that exhibit altered stability and replication.
Inselburg J. Inselburg J. J Bacteriol. 1981 Sep;147(3):962-71. doi: 10.1128/jb.147.3.962-971.1981. J Bacteriol. 1981. PMID: 6268615 Free PMC article. - Complementation of replication-deficient deletion derivatives of plasmid mini-F.
Ebbers J, Eichenlaub R. Ebbers J, et al. J Bacteriol. 1981 Sep;147(3):736-43. doi: 10.1128/jb.147.3.736-743.1981. J Bacteriol. 1981. PMID: 6268604 Free PMC article.
References
- Biochem Biophys Res Commun. 1970 Dec 24;41(6):1538-44 - PubMed
- Annu Rev Microbiol. 1957;11:7-22 - PubMed
MeSH terms
LinkOut - more resources
Full Text Sources