Population analysis of the deinduction kinetics of galactose long-term adaptation mutants of yeast - PubMed (original) (raw)

Population analysis of the deinduction kinetics of galactose long-term adaptation mutants of yeast

S Tsuyumu et al. Proc Natl Acad Sci U S A. 1973 Mar.

Abstract

By use of a selective galactose agar medium containing ethidium bromide, a population analysis of the deinduction kinetics of yeast galactose long-term adaptation mutants (gal 3) has been done. It was first determined that the gal 3 mutation is specific to the yeast galactose system and that induced cultures of gal 3 strains are capable of growth on galactose agar medium containing ethidium bromide, whereas noninduced cultures are not. Population analyses of induced gal 3 strains under going deinduction in the absence of galactose demonstrate that a minimum number of five induction units per cell are required for induction of the galactose system. It is concluded that: these induction units are actively synthesized only in the presence of inducer and are diluted out through cell division; they are stable under nongrowing conditions; they are heterogeneous in nature; at most two of the five minimum units are products of the gal 2 locus; and the other units may be three of one type, one of one type and two of another, or one each of three different types.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1950 Nov;36(11):591-606 - PubMed
    1. Cold Spring Harb Symp Quant Biol. 1951;16:87-98 - PubMed
    1. J Bacteriol. 1959 Nov;78:613-23 - PubMed
    1. J Bacteriol. 1971 Jul;107(1):377-81 - PubMed
    1. Proc Natl Acad Sci U S A. 1952 Jul;38(7):583-92 - PubMed

MeSH terms

Substances

LinkOut - more resources