Use of DNA polymerase I primed by a synthetic oligonucleotide to determine a nucleotide sequence in phage fl DNA - PubMed (original) (raw)

Use of DNA polymerase I primed by a synthetic oligonucleotide to determine a nucleotide sequence in phage fl DNA

F Sanger et al. Proc Natl Acad Sci U S A. 1973 Apr.

Abstract

A sequence of 50 residues in f1 DNA has been determined by the extension of a chemically synthesized octadeoxyribonucleotide by Escherichia coli DNA polymerase I, with radioactive nucleoside triphosphates and f1 DNA template. The polymerized product was synthesized either in the presence of manganese and a mixture of ribo- and deoxyribotriphosphates or in a magnesium-containing reaction with one or more of the four triphosphates absent. The sequence determination depended largely on fractionation of the polymerized products by two-dimensional "homochromatography." This approach and the techniques for the subsequent sequence analysis should be of general use for determining other sequences of DNA. Several features of this sequence suggest that it is located in an intercistronic region of f1 DNA.

PubMed Disclaimer

Similar articles

• The use of primed synthesis by DNA polymerase I to study an intercistronic sequence of phiX-174 DNA.
  Donelson JE, Barrell BG, Weith HL. Donelson JE, et al. Eur J Biochem. 1975 Oct 15;58(2):383-95. doi: 10.1111/j.1432-1033.1975.tb02385.x. Eur J Biochem. 1975. PMID: 1102305
• The 3'-terminal nucleotide sequences of lambda DNA.
  Brezinski DP, Wang JC. Brezinski DP, et al. Biochem Biophys Res Commun. 1973 Jan 23;50(2):398-404. doi: 10.1016/0006-291x(73)90854-1. Biochem Biophys Res Commun. 1973. PMID: 4569877 No abstract available.
• Nucleotide sequence analysis of DNA. IV. Complete nucleotide sequence of the left-hand cohesive end of coliphage 186 DNA.
  Padmanabhan R, Wu R. Padmanabhan R, et al. J Mol Biol. 1972 Apr 14;65(3):447-67. doi: 10.1016/0022-2836(72)90201-x. J Mol Biol. 1972. PMID: 4554112 No abstract available.
• The 3'-terminal nucleotide sequences of T7 DNA.
  Englund PT. Englund PT. J Mol Biol. 1972 May 14;66(2):209-24. doi: 10.1016/0022-2836(72)90474-3. J Mol Biol. 1972. PMID: 5038449 No abstract available.
• Determination of a nucleotide sequence in bacteriophage f1 DNA by primed synthesis with DNA polymerase.
  Sanger F, Donelson JE, Coulson AR, Kössel H, Fischer D. Sanger F, et al. J Mol Biol. 1974 Dec 5;90(2):315-33. doi: 10.1016/0022-2836(74)90376-3. J Mol Biol. 1974. PMID: 4616099 No abstract available.

Cited by

• Nucleotide sequence in the promoter region of the Escherichia coli tyrosine tRNA gene.
  Sekiya T, Khorana HG. Sekiya T, et al. Proc Natl Acad Sci U S A. 1974 Aug;71(8):2978-82. doi: 10.1073/pnas.71.8.2978. Proc Natl Acad Sci U S A. 1974. PMID: 4606851 Free PMC article.
• The use of nuclease P1 in sequence analysis of end group labeled RNA.
  Silberklang M, Gillum AM, RajBhandary UL. Silberklang M, et al. Nucleic Acids Res. 1977 Dec;4(12):4091-108. doi: 10.1093/nar/4.12.4091. Nucleic Acids Res. 1977. PMID: 202925 Free PMC article.
• Synthesis and hybridization of dodecadeoxyribonucleotides containing a fluorescent pyridopyrimidine deoxynucleoside.
  Inoue H, Imura A, Ohtsuka E. Inoue H, et al. Nucleic Acids Res. 1985 Oct 11;13(19):7119-28. doi: 10.1093/nar/13.19.7119. Nucleic Acids Res. 1985. PMID: 4059054 Free PMC article.
• Nucleotide sequence of HS-beta satellite DNA from kangaroo rat Dipodomys ordii.
  Fry K, Poon R, Whitcome P, Idriss J, Salser W, Mazrimas J, Hatch F. Fry K, et al. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2642-6. doi: 10.1073/pnas.70.9.2642. Proc Natl Acad Sci U S A. 1973. PMID: 4517675 Free PMC article.
• Extensive chromosome homoeology among Brassiceae species were revealed by comparative genetic mapping with high-density EST-based SNP markers in radish (Raphanus sativus L.).
  Li F, Hasegawa Y, Saito M, Shirasawa S, Fukushima A, Ito T, Fujii H, Kishitani S, Kitashiba H, Nishio T. Li F, et al. DNA Res. 2011 Oct;18(5):401-11. doi: 10.1093/dnares/dsr027. Epub 2011 Aug 4. DNA Res. 2011. PMID: 21816873 Free PMC article.

References

1. 1. J Mol Biol. 1965 Sep;13(2):373-98 - PubMed
2. 1. Proc Natl Acad Sci U S A. 1963 Oct;50:664-72 - PubMed
3. 1. Hoppe Seylers Z Physiol Chem. 1969 Sep;350(9):1047-66 - PubMed
4. 1. Biochim Biophys Acta. 1969 Oct 22;190(2):548-50 - PubMed
5. 1. Nature. 1969 Dec 13;224(5224):1083-6 - PubMed

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Other Literature Sources

  • The Lens - Patent Citations Database