Restriction of gene expression in B lymphocytes and their progeny. I. Commitment to immunoglobulin allotype - PubMed (original) (raw)

Restriction of gene expression in B lymphocytes and their progeny. I. Commitment to immunoglobulin allotype

P P Jones et al. J Exp Med. 1974.

Abstract

Lymphocytes from b(5)/b(9) rabbits were stained in suspension with fluorescent antiallotype antibody reagents to selectively label with fluorescent molecules those cells bearing membrane immunoglobulin (Ig) of the b5 or b9 allotype. After staining, the cells were separated by the fluorescence-activated cell sorter into populations markedly enriched in cells bearing b5 or b9 membrane Ig or totally depleted of cells with detectable membrane Ig. The potential of these separated cells to give rise to Ig-synthesizing plasma cells either in vivo after transfer into irradiated recipients or in vitro during culture in the presence of phytohemagglutinin or pokeweed mitogen was assessed by immunofluorescence. The relative proportion of b5 and b9 cytoplasmic Ig-stained cells (CSC) arising from the separated cells was determined to test directly whether B lymphocytes and their progeny are committed to the synthesis of Ig of one allotype. It was found that b5- and b9-bearing cells gave rise almost exclusively to b5- and b9-producing plasma cells, respectively, in both the in vivo and in vitro assay systems. Most of these CSC were probably not derived from previously existing CSC but arose as the result of the differentiation of lymphocytes with membrane Ig. When cell populations totally depleted of Ig-bearing lymphocytes were cultured, very few CSC were found, indicating that the majority of immediate precursors of CSC have membrane Ig. These results suggest that individual B cell clones are phenotypically restricted to the expression of immunoglobulin genes on one chromosome; the significance of this clonal allelic exclusion is discussed.

PubMed Disclaimer

Cited by

B cell receptor editing in tolerance and autoimmunity.
Luning Prak ET, Monestier M, Eisenberg RA. Luning Prak ET, et al. Ann N Y Acad Sci. 2011 Jan;1217:96-121. doi: 10.1111/j.1749-6632.2010.05877.x. Epub 2011 Jan 5. Ann N Y Acad Sci. 2011. PMID: 21251012 Free PMC article. Review.
Origin and fate of IgE-bearing lymphocytes. I. Peyer's patches as differentiation site of cells. Simultaneously bearing IgA and IgE.
Durkin HG, Bazin H, Waksman BH. Durkin HG, et al. J Exp Med. 1981 Sep 1;154(3):640-8. doi: 10.1084/jem.154.3.640. J Exp Med. 1981. PMID: 6974216 Free PMC article.
Restriction of gene expression in B lymphocytes and their progeny. II. Commitment to immunoglobulin heavy chain isotype.
Jones PP, Craig SW, Cebra JJ, Herzenberg LA. Jones PP, et al. J Exp Med. 1974 Aug 1;140(2):452-69. doi: 10.1084/jem.140.2.452. J Exp Med. 1974. PMID: 4603013 Free PMC article.
Restriction of gene expression in B lymphocytes and their progeny. III. Endogenous IgA and IgM on the membranes of different plasma cell precursors.
Jones PP, Cebra JJ. Jones PP, et al. J Exp Med. 1974 Oct 1;140(4):966-76. doi: 10.1084/jem.140.4.966. J Exp Med. 1974. PMID: 4139228 Free PMC article.
The Fc receptor on thymus-derived lymphocytes. I. Detection of a subpopulation of murine T lymphocytes bearing the Fc receptor.
Stout RD, Herzenberg LA. Stout RD, et al. J Exp Med. 1975 Sep 1;142(3):611-21. doi: 10.1084/jem.142.3.611. J Exp Med. 1975. PMID: 1100762 Free PMC article.

References

1. J Immunol. 1967 Jan;98(1):17-30 - PubMed
1. Proc Natl Acad Sci U S A. 1965 Sep;54(3):864-9 - PubMed
1. Clin Exp Immunol. 1971 Oct;9(4):483-98 - PubMed
1. Immunology. 1964 Sep;7:500-6 - PubMed
1. J Exp Med. 1973 Jan 1;137(1):22-31 - PubMed

MeSH terms

Substances

LinkOut - more resources

Full Text Sources

Restriction of gene expression in B lymphocytes and their progeny. I. Commitment to immunoglobulin allotype - PubMed (original) (raw)