Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA - PubMed (original) (raw)
Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA
V Hershfield et al. Proc Natl Acad Sci U S A. 1974 Sep.
Abstract
DNA fragments obtained from EcoRI endonuclease digestion of bacteriophage varphi80pt190 (trp(+)) and the plasmid ColE1 were covalently joined with polynucleotide ligase. Transformation of Escherichia coli trp(-) strains to tryptophan independence with the recombined DNA selected for reconstituted ColE1 plasmids containing the tryptophan operon and the varphi80 immunity region. Similarly, an EcoRI endonuclease generated fragment of plasmid pSC105 DNA containing the genetic determinant of kanamycin resistance was inserted into the ColE1 plasmid and recovered in E. coli. The plasmids containing the trp operon (ColE1-trp) and the kanamycin resistance gene were maintained under logarithmic growth conditions at a level of 25-30 copies per cell and accumulate to the extent of several hundred copies per cell in the presence of chloramphenicol. Cells carrying the ColE1-trp plasmid determined the production of highly elevated levels of trp operon-specific mRNA and tryptophan biosynthetic enzymes.
Similar articles
- Biochemical construction of specific chimeric plasmids from ColE1 DNA and unfractionated Escherichia coli DNA.
Collins CJ, Jackson DA, DeVries AJ. Collins CJ, et al. Proc Natl Acad Sci U S A. 1976 Nov;73(11):3838-42. doi: 10.1073/pnas.73.11.3838. Proc Natl Acad Sci U S A. 1976. PMID: 792875 Free PMC article. - Excision of a DNA sequence determining kanamycin resistance from a ColE1-Km recombinant plasmid.
Bodsch W. Bodsch W. Mol Gen Genet. 1977 Jan 7;150(1):29-36. doi: 10.1007/BF02425322. Mol Gen Genet. 1977. PMID: 319342 - Characterization of a mini-ColC1 plasmid.
Hershfield V, Boyer HW, Chow L, Helinski DR. Hershfield V, et al. J Bacteriol. 1976 Apr;126(1):447-53. doi: 10.1128/jb.126.1.447-453.1976. J Bacteriol. 1976. PMID: 770430 Free PMC article. - [On the in vitro recombination using plasmid or phage DNA (author's transl)].
Matsubara K. Matsubara K. Tanpakushitsu Kakusan Koso. 1975 May;20(6):574-80. Tanpakushitsu Kakusan Koso. 1975. PMID: 1101304 Review. Japanese. No abstract available. - Replication and expression of constructed plasmid chimeras in transformed Escherichia coli--a review.
Cohen SN, Chang AC. Cohen SN, et al. Basic Life Sci. 1975;5A:335-44. doi: 10.1007/978-1-4684-2895-7_46. Basic Life Sci. 1975. PMID: 1103843 Review.
Cited by
- Characterization of novel double-reporter strains of Mycobacterium abscessus for drug discovery: a study in mScarlet.
Bento CM, van Calster K, Piller T, Oliveira GS, de Vooght L, Cappoen D, Cos P, Gomes MS, Silva T. Bento CM, et al. Microbiol Spectr. 2024 Oct 3;12(10):e0036224. doi: 10.1128/spectrum.00362-24. Epub 2024 Aug 27. Microbiol Spectr. 2024. PMID: 39189762 Free PMC article. - Synthetic Biology Toolbox for Nitrogen-Fixing Soil Microbes.
Venkataraman M, Yñigez-Gutierrez A, Infante V, MacIntyre A, Fernandes-Júnior PI, Ané JM, Pfleger B. Venkataraman M, et al. ACS Synth Biol. 2023 Dec 15;12(12):3623-3634. doi: 10.1021/acssynbio.3c00414. Epub 2023 Nov 21. ACS Synth Biol. 2023. PMID: 37988619 - TReSR: A PCR-compatible DNA sequence design method for engineering proteins containing tandem repeats.
Davey JA, Goto NK. Davey JA, et al. PLoS One. 2023 Apr 12;18(4):e0281228. doi: 10.1371/journal.pone.0281228. eCollection 2023. PLoS One. 2023. PMID: 37043448 Free PMC article. - Unlocking the strength of inducible promoters in Gram-negative bacteria.
Carrillo Rincón AF, Farny NG. Carrillo Rincón AF, et al. Microb Biotechnol. 2023 May;16(5):961-976. doi: 10.1111/1751-7915.14219. Epub 2023 Feb 3. Microb Biotechnol. 2023. PMID: 36738130 Free PMC article. - Engineering bacteria to control electron transport altering the synthesis of non-native polymer.
Bennett MR, Jain A, Kovacs K, Hill PJ, Alexander C, Rawson FJ. Bennett MR, et al. RSC Adv. 2021 Dec 21;12(1):451-457. doi: 10.1039/d1ra06403g. eCollection 2021 Dec 20. RSC Adv. 2021. PMID: 35424487 Free PMC article.
References
- Nature. 1965 Nov 27;208(5013):843-9 - PubMed
- Virology. 1967 Feb;31(2):289-95 - PubMed
- J Mol Biol. 1966 Nov 14;21(2):313-34 - PubMed
- J Mol Biol. 1968 Feb 14;31(3):519-40 - PubMed
- Proc Natl Acad Sci U S A. 1968 Jul;60(3):992-9 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials