Protein migration into nuclei. I. Frog oocyte nuclei in vivo accumulate microinjected histones, allow entry to small proteins, and exclude large proteins - PubMed (original) (raw)
Protein migration into nuclei. I. Frog oocyte nuclei in vivo accumulate microinjected histones, allow entry to small proteins, and exclude large proteins
W M Bonner. J Cell Biol. 1975 Feb.
Abstract
A technique is presented which enables one to measure the extent to which a protein enters and accumulates in the nucleus of the frog oocyte. In this method, the protein, labeled with 125-I, is microinjected into the oocyte. After incubation, the oocyte is manually enucleated and the radioactivity in the nucleus and cytoplasm is determined. Using this technique, proteins lighter than 20,000 daltons were found to enter the nucleus and completely equilibrate between the nucleus and cytoplasm within 24 h. The entry of proteins heavier than 69,000 daltons was severely hindered. Histones and histone fractions entered as quickly as other small proteins, but, in contrast to these proteins, they accumulated in the nucleus to different extents, depending on the total amount of histone injected into the oocyte and the identity of the histone. Evidence is presented that histone fractions compete with each other for accumulation in the nucleus.
Similar articles
- Nucleocytoplasmic exchange of macromolecules.
Paine PL, Feldherr CM. Paine PL, et al. Exp Cell Res. 1972 Sep;74(1):81-98. doi: 10.1016/0014-4827(72)90483-1. Exp Cell Res. 1972. PMID: 4342186 No abstract available. - Injected nuclei in frog oocytes:RNA synthesis and protein exchange.
Gurdon JB, Partington GA, De Robertis EM. Gurdon JB, et al. J Embryol Exp Morphol. 1976 Dec;36(3):541-53. J Embryol Exp Morphol. 1976. PMID: 1010978 - Subcellular distribution in Xenopus laevis oocytes of a microinjected poly(A)-binding protein from Artemia salina gastrulae.
De Herdt E, Marbaix G, Tencer R, Slegers H. De Herdt E, et al. Eur J Biochem. 1983 May 16;132(3):623-7. doi: 10.1111/j.1432-1033.1983.tb07409.x. Eur J Biochem. 1983. PMID: 6852017 - Injected amphibian oocytes: a living test tube for the study of eukaryotic gene transcription?
de Robertis EM, Gurdon JB, Partington GA, Mertz JE, Laskey RA. de Robertis EM, et al. Biochem Soc Symp. 1977;(42):181-91. Biochem Soc Symp. 1977. PMID: 339919 Review.
Cited by
- A karyophilic signal sequence in adenovirus type 5 E1A is functional in Xenopus oocytes but not in somatic cells.
Slavicek JM, Jones NC, Richter JD. Slavicek JM, et al. J Virol. 1989 Sep;63(9):4047-50. doi: 10.1128/JVI.63.9.4047-4050.1989. J Virol. 1989. PMID: 2527314 Free PMC article. - The nuclear localization signal of CPSF6 governs post-nuclear import steps of HIV-1 infection.
Rohlfes N, Radhakrishnan R, Singh PK, Bedwell GJ, Engelman AN, Dharan A, Campbell EM. Rohlfes N, et al. bioRxiv [Preprint]. 2024 Jun 24:2024.06.20.599834. doi: 10.1101/2024.06.20.599834. bioRxiv. 2024. PMID: 38979149 Free PMC article. Updated. Preprint. - Identification of a signal for nuclear targeting in platelet-derived-growth-factor-related molecules.
Lee BA, Maher DW, Hannink M, Donoghue DJ. Lee BA, et al. Mol Cell Biol. 1987 Oct;7(10):3527-37. doi: 10.1128/mcb.7.10.3527-3537.1987. Mol Cell Biol. 1987. PMID: 3316980 Free PMC article. - The coming-of-age of nucleocytoplasmic transport in motor neuron disease and neurodegeneration.
Ferreira PA. Ferreira PA. Cell Mol Life Sci. 2019 Jun;76(12):2247-2273. doi: 10.1007/s00018-019-03029-0. Epub 2019 Feb 11. Cell Mol Life Sci. 2019. PMID: 30742233 Free PMC article. Review. - Association with capsid proteins promotes nuclear targeting of simian virus 40 DNA.
Nakanishi A, Clever J, Yamada M, Li PP, Kasamatsu H. Nakanishi A, et al. Proc Natl Acad Sci U S A. 1996 Jan 9;93(1):96-100. doi: 10.1073/pnas.93.1.96. Proc Natl Acad Sci U S A. 1996. PMID: 8552683 Free PMC article.
References
- Exp Cell Res. 1972 Sep;74(1):81-98 - PubMed
- Biochem J. 1969 Jun;113(2):299-305 - PubMed
- J Cell Biol. 1974 Feb;60(2):405-15 - PubMed
- Annu Rev Biochem. 1971;40:279-314 - PubMed
- J Cell Sci. 1969 Sep;5(2):333-49 - PubMed