Production and purification of acid protease from the thermophilic fungus, Penicillium duponti K1014 - PubMed (original) (raw)
Production and purification of acid protease from the thermophilic fungus, Penicillium duponti K1014
H Hashimoto et al. Appl Microbiol. 1973 Apr.
Abstract
A thermophilic fungus, Penicillium duponti K1014, produced an acid protease in a submerged culture. Maximum enzyme production, in a 30-liter fermentor at a preselected optimum growth temperature of 50 C, occurred after 3 days. The productivity of the enzyme was associated with changes of viscosity and pH of culture broth during the growth. The acid protease was isolated from the culture filtrates and was purified ninefold by alcohol precipitation, column chromatography on O-(diethylaminoethyl)cellulose, batchwise treatment with O-(carboxymethyl)cellulose, and by gel filtration. The purified enzyme was homogeneous on disk electrophoresis and sedimentation analysis. The sedimentation constant s(20.w), calculated at a concentration of 0.64%, was 3.98S.
Similar articles
- Some properties of acid protease from the thermophilic fungus, Penicillium duponti K1014.
Hashimoto H, Iwaasa T, Yokotsuka T. Hashimoto H, et al. Appl Microbiol. 1973 Apr;25(4):578-83. doi: 10.1128/am.25.4.578-583.1973. Appl Microbiol. 1973. PMID: 4699217 Free PMC article. - Thermostable acid protease produced by Penicillium duponti K1014, a true thermophilic fungus newly isolated from compost.
Hashimoto H, Iwaasa T, Yokotsuka T. Hashimoto H, et al. Appl Microbiol. 1972 Dec;24(6):986-92. doi: 10.1128/am.24.6.986-992.1972. Appl Microbiol. 1972. PMID: 4650601 Free PMC article. - Purification and properties of the thermostable acid protease of Penicillium duponti.
Emi S, Myers DV, Iacobucci GA. Emi S, et al. Biochemistry. 1976 Feb 24;15(4):842-8. doi: 10.1021/bi00649a018. Biochemistry. 1976. PMID: 2287 - [Proteolytic system in Penicillium roqueforti. 2. Purification and properties of acid protease].
Zevaco C, Hermier J, Gripon JC. Zevaco C, et al. Biochimie. 1973;55(11):1353-60. Biochimie. 1973. PMID: 4790849 French. No abstract available. - Purification of an extracellular proteinase from Penicillium notatum.
Makonnen B, Porath J. Makonnen B, et al. Eur J Biochem. 1968 Nov;6(3):425-31. doi: 10.1111/j.1432-1033.1968.tb00464.x. Eur J Biochem. 1968. PMID: 5726849 No abstract available.
Cited by
- Some properties of acid protease from the thermophilic fungus, Penicillium duponti K1014.
Hashimoto H, Iwaasa T, Yokotsuka T. Hashimoto H, et al. Appl Microbiol. 1973 Apr;25(4):578-83. doi: 10.1128/am.25.4.578-583.1973. Appl Microbiol. 1973. PMID: 4699217 Free PMC article. - Thermophilic fungi: their physiology and enzymes.
Maheshwari R, Bharadwaj G, Bhat MK. Maheshwari R, et al. Microbiol Mol Biol Rev. 2000 Sep;64(3):461-88. doi: 10.1128/MMBR.64.3.461-488.2000. Microbiol Mol Biol Rev. 2000. PMID: 10974122 Free PMC article. Review.
References
- Nature. 1966 Apr 9;210(5032):220 - PubMed
- Appl Microbiol. 1967 Nov;15(6):1309-12 - PubMed
- J Bacteriol. 1968 Apr;95(4):1407-14 - PubMed
- Genetics. 1969 Sep;63(1):75-92 - PubMed
- Can J Biochem. 1970 Apr;48(4):425-31 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources