Affinity chromatography on immobilized hyaluronate and its application to the isolation of hyaluronate binding properties from cartilage - PubMed (original) (raw)

Affinity chromatography on immobilized hyaluronate and its application to the isolation of hyaluronate binding properties from cartilage

A Tengblad. Biochim Biophys Acta. 1979.

Abstract

Partially degraded hyaluronate was coupled to AH-Sepharose 4B using carbodiimide. Approximately 1 mg of hyaluronate was incorporated per ml of wet gel. The derivatized gel was used to purify components of the hyaluronate-proteoglycan complex of cartilage. Two link-proteins were isolated from a crude cartilage extract by affinity binding to the gel and eluted with 4 M guanidinium chloride. By the same procedure one link-protein and the globular portion of the proteoglycan monomer were isolated from a trypsin-treated cartilage extract and were separated from each other by subsequent gel chromatography on Sepharose 6B and Sephacryl S-200. The affinity technique was also used for the preparation of these proteins labelled with dansyl groups.

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