Nonsense suppression in a multiauxotrophic derivative of Escherichia coli 15T-: identification and consequences of an amber triplet in the deoxyribomutase gene - PubMed (original) (raw)
Nonsense suppression in a multiauxotrophic derivative of Escherichia coli 15T-: identification and consequences of an amber triplet in the deoxyribomutase gene
R C Bockrath et al. J Bacteriol. 1968 Jul.
Abstract
Previously, arginine revertants of Escherichia coli WWU, a derivative of E. coli 15T(-), have been subdivided by two independent methods: (i) the streak morphology on nutrient agar, and (ii) the pattern of phage growth using amber and ochre mutants of bacteriophage T4. In the first assay, revertants were subdivided into two classes according to the appearance of streaks after incubation on nutrient agar, a thick, even line of growth defining normal revertants and a thin, irregular line defining aberrant revertants. In the second assay, revertants were classified by the suppressors they contained. The present work demonstrates that revertants containing an amber suppressor show the aberrant morphology and are also able to catabolize thymidine for energy and carbon. This is in contrast to the parent WWU containing no suppressor, which shows a normal morphology and cannot utilize thymidine as an energy source. Revertants containing no suppressor, isolated specifically for their ability to catabolize thymidine, show an aberrant morphology. Together, these results indicate that the aberrant morphology results from suppression of an amber triplet in a gene of the thymidine catabolic pathway. Enzyme assays show the amber triplet to be in the gene specifying deoxyribomutase. It is suggested that the aberrant arginine revertants are analogous to high thymine-requiring mutants and that, in general, high and low thymine-requiring mutants differ from one another in their ability to catabolize deoxyribose-1-phosphate.
Similar articles
- An amber suppressor of Escherichia coli strain KO1.
Aoi T, Watanabe I. Aoi T, et al. Jpn J Microbiol. 1975 Jun;19(3):193-9. Jpn J Microbiol. 1975. PMID: 1100889 - Characterization of revertants of E. coli WU36-10 and WP2 using amber mutants and an ochre mutant of bacteriphage T4.
Osborn M, Person S. Osborn M, et al. Mutat Res. 1967 Jul-Aug;4(4):504-7. doi: 10.1016/0027-5107(67)90013-9. Mutat Res. 1967. PMID: 4862436 No abstract available. - A functional requirement for modification of the wobble nucleotide in tha anticodon of a T4 suppressor tRNA.
Colby DS, Schedl P, Guthrie C. Colby DS, et al. Cell. 1976 Nov;9(3):449-63. doi: 10.1016/0092-8674(76)90090-8. Cell. 1976. PMID: 791507 - Bacterial genetic factors controlling the suppression of T4 phage amber mutants. I. Suppression patterns of a collection of E. coli strains.
Krieg RH, Stent GS. Krieg RH, et al. Mol Gen Genet. 1968;103(3):274-93. doi: 10.1007/BF00273699. Mol Gen Genet. 1968. PMID: 4890344 No abstract available. - Search for revertants of the glutamine mischarging mutans of Escherichia coli su+3 tyrosine suppressor tRNA that are able to insert tyrosine at the site of amber mutation.
Celis JE, Squire M, Kaltoft K, Riisom E. Celis JE, et al. Nucleic Acids Res. 1977 Aug;4(8):2799-809. doi: 10.1093/nar/4.8.2799. Nucleic Acids Res. 1977. PMID: 410001 Free PMC article.
Cited by
- Effect of Selenomethionine on Growth of Escherichia coli and Bacillus megaterium.
Wu M, Wachsman JT. Wu M, et al. J Bacteriol. 1970 Dec;104(3):1393-6. doi: 10.1128/jb.104.3.1393-1396.1970. J Bacteriol. 1970. PMID: 16559120 Free PMC article. - Physiological Streptomycin Resistance in a Multiauxotroph of Escherichia coli Strain 15 T.
Carlson K, Bockrath RC. Carlson K, et al. J Bacteriol. 1970 Dec;104(3):1294-8. doi: 10.1128/jb.104.3.1294-1298.1970. J Bacteriol. 1970. PMID: 16559106 Free PMC article. - Methylated bases of Bacillus megaterium KM nucleic acids: comparison with Escherichia coli.
Wachsman JT, Irwin V. Wachsman JT, et al. J Bacteriol. 1970 Nov;104(2):814-8. doi: 10.1128/jb.104.2.814-818.1970. J Bacteriol. 1970. PMID: 4992370 Free PMC article. - Transcription spectra in E. coli growing rapidly or slowly.
Newman CN, Bockrath RC. Newman CN, et al. Experientia. 1977 Jul 15;33(7):893-4. doi: 10.1007/BF01951266. Experientia. 1977. PMID: 330198 No abstract available.
References
- J Mol Biol. 1965 Dec;14(2):528-33 - PubMed
- J Gen Microbiol. 1965 Dec;41(3):321-33 - PubMed
- J Mol Biol. 1965 Nov;14(1):167-78 - PubMed
- J Bacteriol. 1967 Mar;93(3):1188-9 - PubMed
- J Mol Biol. 1967 Jun 28;26(3):437-47 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources