An active site peptide from pepsin C - PubMed (original) (raw)
- PMID: 4942834
- PMCID: PMC1176901
- DOI: 10.1042/bj1230075
An active site peptide from pepsin C
J Kay et al. Biochem J. 1971 Jun.
Abstract
Porcine pepsin C is inactivated rapidly and irreversibly by diazoacetyl-dl-norleucine methyl ester in the presence of cupric ions at pH values above 4.5. The inactivation is specific in that complete inactivation accompanies the incorporation of 1mol of inhibitor residue/mol of enzyme and evidence has been obtained to suggest that the reaction occurs with an active site residue. The site of reaction is the beta-carboxyl group of an aspartic acid residue in the sequence Ile-Val-Asp-Thr. This sequence is identical with the active-site sequence in pepsin and the significance of this in terms of the different activities of the two enzymes is discussed.
Similar articles
- Bovine pepsinogens and pepsins. The sequence around a reactive aspartyl residue.
Meitner PA. Meitner PA. Biochem J. 1971 Oct;124(4):673-6. doi: 10.1042/bj1240673. Biochem J. 1971. PMID: 4943483 Free PMC article. - An aspartic acid residue at the active site of pepsin. The isolation and sequence of the heptapeptide.
Bayliss RS, Knowles JR, Wybrandt GB. Bayliss RS, et al. Biochem J. 1969 Jun;113(2):377-86. doi: 10.1042/bj1130377. Biochem J. 1969. PMID: 4897201 Free PMC article. - Thyroid acid proteinase. Properties and inactivation by diazoacetyl-norleucine methyl ester.
Smith GD, Murray MA, Nichol LW, Trikojus VM. Smith GD, et al. Biochim Biophys Acta. 1969 Feb 11;171(2):288-98. doi: 10.1016/0005-2744(69)90162-4. Biochim Biophys Acta. 1969. PMID: 4886478 No abstract available. - [Acid proteases (author's transl)].
Morihara K. Morihara K. Tanpakushitsu Kakusan Koso. 1977 Oct;22(11):1259-70. Tanpakushitsu Kakusan Koso. 1977. PMID: 339278 Review. Japanese. No abstract available.
Cited by
- Bovine pepsinogens and pepsins. The sequence around a reactive aspartyl residue.
Meitner PA. Meitner PA. Biochem J. 1971 Oct;124(4):673-6. doi: 10.1042/bj1240673. Biochem J. 1971. PMID: 4943483 Free PMC article. - Kinetics of irreversible enzyme inhibition by an unstable inhibitor.
Rakitzis ET. Rakitzis ET. Biochem J. 1974 Aug;141(2):601-3. doi: 10.1042/bj1410601. Biochem J. 1974. PMID: 4616685 Free PMC article. - Kinetics of protein modification, and/or enzyme inactivation, reactions by an unstable modifying agent.
Rakitzis ET. Rakitzis ET. Biochem J. 1987 Sep 15;246(3):803-5. doi: 10.1042/bj2460803. Biochem J. 1987. PMID: 3689335 Free PMC article. No abstract available. - The catalytic activity of pig pepsin C towards small synthetic substrates.
Auffret CA, Ryle AP. Auffret CA, et al. Biochem J. 1979 Apr 1;179(1):239-46. doi: 10.1042/bj1790239. Biochem J. 1979. PMID: 38772 Free PMC article. - The effect of acid proteinase inhibitors on chicken pepsin.
Llewellin JM, Green ML. Llewellin JM, et al. Biochem J. 1975 Nov;151(2):319-26. doi: 10.1042/bj1510319. Biochem J. 1975. PMID: 3165 Free PMC article.
References
- J Biol Chem. 1969 Jan 10;244(1):154-60 - PubMed
- J Am Chem Soc. 1967 Aug 2;89(16):4204-8 - PubMed
- Biochem J. 1960 Apr;75:145-50 - PubMed
- Anal Biochem. 1966 Mar;14(3):429-33 - PubMed
- J Am Chem Soc. 1968 Jan 17;90(2):479-86 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources