Development of competence in the Bacillus subtilis transformation system - PubMed (original) (raw)
Development of competence in the Bacillus subtilis transformation system
K F Bott et al. J Bacteriol. 1967 Sep.
Abstract
Competence in Bacillus subtilis, assayed by the ability of cells to be transformed with bacterial deoxyribonucleic acid (DNA) or transfected by phage DNA, has been shown to occur in a single semisynthetic medium with peak activity occurring 3 hr after the cessation of logarithmic growth. No step-down conditions or culture manipulations were necessary for routine transfection of 1% of the population. The results demonstrate that bacteriophage DNA is a valid assay for studying the development of competence in B. subtilis. Predictions of workers using transforming bacterial DNA, who have suggested that competence in B. subtilis is associated with a specific phase of growth, are substantiated. The peak of competence is not affected by marked differences in the rate of growth during the logarithmic phase. The effect on development of competence by this procedure of some components (including casein hydrolysate, tryptophan, and histidine) which were routinely included in the transformation medium by other investigators has been determined by use of infectious phage DNA as an assay. We have demonstrated that tryptophan, as well as histidine, increases the transformation frequency-even in strains which do not have auxotrophic demands for these components. Glutamic acid and alanine depress optimal levels of transfection.
Similar articles
- Relationship between competence for transfection and for transformation.
Riva S, Polsinelli M. Riva S, et al. J Virol. 1968 Jun;2(6):587-93. doi: 10.1128/JVI.2.6.587-593.1968. J Virol. 1968. PMID: 4177104 Free PMC article. - [Relationship between the instability in saline and the competence for genetic transformation in Bacillus subtilis].
Grunow R. Grunow R. Z Allg Mikrobiol. 1973;13(8):639-45. doi: 10.1002/jobm.3630130802. Z Allg Mikrobiol. 1973. PMID: 4205483 German. No abstract available. - Nutritional factors influencing the development of competence in the Bacillus subtilis transformation system.
Wilson GA, Bott KF. Wilson GA, et al. J Bacteriol. 1968 Apr;95(4):1439-49. doi: 10.1128/jb.95.4.1439-1449.1968. J Bacteriol. 1968. PMID: 4967198 Free PMC article. - Relationship between competence for transformation of Bacillus subtilis with native and single-stranded deoxyribonucleic acid.
Tevethia MJ, Caudill CP. Tevethia MJ, et al. J Bacteriol. 1971 Jun;106(3):808-11. doi: 10.1128/jb.106.3.808-811.1971. J Bacteriol. 1971. PMID: 4997539 Free PMC article. - Transformation and transduction in recombination-defective mutants of Bacillus subtilis.
Hoch JA, Barat M, Anagnostopoulos C. Hoch JA, et al. J Bacteriol. 1967 Jun;93(6):1925-37. doi: 10.1128/jb.93.6.1925-1937.1967. J Bacteriol. 1967. PMID: 4960898 Free PMC article.
Cited by
- Signal Peptidase-Mediated Cleavage of the Anti-σ Factor RsiP at Site 1 Controls σP Activation and β-Lactam Resistance in Bacillus thuringiensis.
Nauta KM, Ho TD, Ellermeier CD. Nauta KM, et al. mBio. 2021 Feb 22;13(1):e0370721. doi: 10.1128/mbio.03707-21. Epub 2022 Feb 15. mBio. 2021. PMID: 35164554 Free PMC article. - Anthranilate Acts as a Signal to Modulate Biofilm Formation, Virulence, and Antibiotic Tolerance of Pseudomonas aeruginosa and Surrounding Bacteria.
Hwang HJ, Li XH, Kim SK, Lee JH. Hwang HJ, et al. Microbiol Spectr. 2022 Feb 23;10(1):e0146321. doi: 10.1128/spectrum.01463-21. Epub 2022 Jan 12. Microbiol Spectr. 2022. PMID: 35019684 Free PMC article. - Simultaneous Multiplex Genome Engineering via Accelerated Natural Transformation in Bacillus subtilis.
Deng A, Sun Z, Wang T, Cui D, Li L, Liu S, Huang F, Wen T. Deng A, et al. Front Microbiol. 2021 Aug 17;12:714449. doi: 10.3389/fmicb.2021.714449. eCollection 2021. Front Microbiol. 2021. PMID: 34484154 Free PMC article. - High-level extracellular production of recombinant nattokinase in Bacillus subtilis WB800 by multiple tandem promoters.
Liu Z, Zheng W, Ge C, Cui W, Zhou L, Zhou Z. Liu Z, et al. BMC Microbiol. 2019 May 7;19(1):89. doi: 10.1186/s12866-019-1461-3. BMC Microbiol. 2019. PMID: 31064343 Free PMC article. - The Anti-sigma Factor RsiV Is a Bacterial Receptor for Lysozyme: Co-crystal Structure Determination and Demonstration That Binding of Lysozyme to RsiV Is Required for σV Activation.
Hastie JL, Williams KB, Bohr LL, Houtman JC, Gakhar L, Ellermeier CD. Hastie JL, et al. PLoS Genet. 2016 Sep 7;12(9):e1006287. doi: 10.1371/journal.pgen.1006287. eCollection 2016 Sep. PLoS Genet. 2016. PMID: 27602573 Free PMC article.
References
- Biochim Biophys Acta. 1966 Jul 20;123(1):56-65 - PubMed
- J Bacteriol. 1961 May;81(5):741-6 - PubMed
- Nature. 1965 Jul 3;207(992):104-5 - PubMed
- J Bacteriol. 1963 Sep;86:392-400 - PubMed
- J Biol Chem. 1966 Aug 10;241(15):3462-7 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources