Isolation and characterization of a cloned rat insulin gene - PubMed (original) (raw)
Isolation and characterization of a cloned rat insulin gene
B Cordell et al. Cell. 1979 Oct.
Abstract
The two nonallelic genes, insulin I and II of the rat, are separated by at least 7 kb of DNA. There is no obvious similarity in the sequence organization surrounding each gene, although the coding regions of the genes themselves share extensive homology. In three strains of rat, the insulin II gene lies predominantly on a 4.0 kb Eco RI restriction fragment, whereas the insulin I gene is located on a 9.4 kb Eco RI fragment in the Hooded strain, on a 7.2 kb Eco RI fragment in the Osborne-Mendel strain, and on both a 9.4 and a 7.2 kb Eco RI fragment in Sprague-Dawley rats. The 9.4 kb Eco RI fragment from Hooded rat DNA was isolated using the lambda cloning system, and the nucleotide sequence of this isolated rat insulin I gene and adjacent regions was determined. A translation in one frame of the sequence of the cloned gene confirms the protein sequence determined for rat pre-proinsulin I. The coding region of this gene lacks intervening sequences, although a presumptive intervening sequence of 119 bp is located in the 5 untranslated region preceding the prehormone sequence. The junctions around the 119 bp segment are identical to those which flank intervening sequences of other eucaryotic genes, AGGT. The site of polyadenylation was determined by direct sequence comparison with rat insulin cDNA clones, and a potential 5 "capping" site is proposed. A DNA sequence preceding this 5 "capping" site in the rat insulin I gene, TATAAAGC, is homologous to corresponding regions in other eucaryotic genes that have been proposed as putative promoter sites for the initiation of transcription.
Similar articles
- Isolation and characterization of cloned human fetal globin genes.
Ramirez F, Burns AL, Mears JG, Spence S, Starkman D, Bank A. Ramirez F, et al. Nucleic Acids Res. 1979 Nov 10;7(5):1147-62. doi: 10.1093/nar/7.5.1147. Nucleic Acids Res. 1979. PMID: 514810 Free PMC article. - Structure of the rat prolactin gene.
Gubbins EJ, Maurer RA, Lagrimini M, Erwin CR, Donelson JE. Gubbins EJ, et al. J Biol Chem. 1980 Sep 25;255(18):8655-62. J Biol Chem. 1980. PMID: 6251061 - A complete immunoglobulin gene is created by somatic recombination.
Brack C, Hirama M, Lenhard-Schuller R, Tonegawa S. Brack C, et al. Cell. 1978 Sep;15(1):1-14. doi: 10.1016/0092-8674(78)90078-8. Cell. 1978. PMID: 100225 - Genomic organization of rat rDNA.
Chikaraishi DM, Buchanan L, Danna KJ, Harrington CA. Chikaraishi DM, et al. Nucleic Acids Res. 1983 Sep 24;11(18):6437-52. doi: 10.1093/nar/11.18.6437. Nucleic Acids Res. 1983. PMID: 6312424 Free PMC article. - The ovalbumin gene: alleles created by mutations in the intervening sequences of the natural gene.
Lai EC, Woo SL, Dugaiczyk A, O'Malley BW. Lai EC, et al. Cell. 1979 Jan;16(1):201-11. doi: 10.1016/0092-8674(79)90201-0. Cell. 1979. PMID: 421270
Cited by
- Effect of transcription factor MEOX on insulin gene expression in glucagon-like peptide 1-secreting cells.
Ryu GR, Bae D, Uddin S, Meah MS, Ahmad W, Silvano KJ, Ahn G, Cha JY, Lee E, Song KH, Kim WY, Kim MG. Ryu GR, et al. In Vitro Cell Dev Biol Anim. 2024 Aug 13. doi: 10.1007/s11626-024-00964-6. Online ahead of print. In Vitro Cell Dev Biol Anim. 2024. PMID: 39138833 - Proinsulin Shares a Motif with Interleukin-1α (IL-1α) and Induces Inflammatory Cytokine via Interleukin-1 Receptor 1.
Lee S, Kim E, Jhun H, Hong J, Kwak A, Jo S, Bae S, Lee J, Kim B, Lee J, Youn S, Kim S, Kim M, Kim H, Lee Y, Choi DK, Kim YS, Kim S. Lee S, et al. J Biol Chem. 2016 Jul 8;291(28):14620-7. doi: 10.1074/jbc.M116.731026. Epub 2016 May 16. J Biol Chem. 2016. PMID: 27226621 Free PMC article. - The glucose-induced synthesis of insulin in liver.
Ghosh R, Karmohapatra SK, Bhattacharya G, Kumar Sinha A. Ghosh R, et al. Endocrine. 2010 Oct;38(2):294-302. doi: 10.1007/s12020-010-9388-3. Epub 2010 Oct 23. Endocrine. 2010. PMID: 20972731 - L-selectin can facilitate metastasis to lymph nodes in a transgenic mouse model of carcinogenesis.
Qian F, Hanahan D, Weissman IL. Qian F, et al. Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):3976-81. doi: 10.1073/pnas.061633698. Epub 2001 Mar 13. Proc Natl Acad Sci U S A. 2001. PMID: 11274419 Free PMC article. - Evidence for biosynthesis of preproinsulin in gut of rat.
Kendzierski KS, Pansky B, Budd GC, Saffran M. Kendzierski KS, et al. Endocrine. 2000 Dec;13(3):353-9. doi: 10.1385/ENDO:13:3:353. Endocrine. 2000. PMID: 11216648
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases