Identification of marrow-derived and thymus-derived small lymphocytes in the lymphoid tissue and thoracic duct lymph of normal rats - PubMed (original) (raw)
Identification of marrow-derived and thymus-derived small lymphocytes in the lymphoid tissue and thoracic duct lymph of normal rats
J C Howard et al. J Exp Med. 1972.
Abstract
These experiments show that small lymphocytes from the thoracic duct of rats are normally a mixture of thymus-derived and marrow-derived cells, and define the traffic areas in lymphoid tissues through which the two populations recirculate. Thoracic duct lymphocytes were labeled in vitro with uridine-(3)H and their histological distribution in the lymphoid tissues of normal recipients was demonstrated by radioautography. Labeled lymphocytes occupied two adjacent areas distinguished by a marked difference in the intensity of labeling; heavily labeled cells were found in thymus-dependent traffic areas of lymphocyte recirculation, while lightly labeled cells localized in the thymus-independent follicular areas around germinal centers. A corresponding heterogeneity of uridine uptake among small lymphocytes from normal donors was demonstrated by sedimentation at 1 g; slowly sedimenting cells incorporated little uridine and localized in follicular areas after transfusion while rapidly sedimenting cells incorporated more uridine and localized in thymus-dependent areas after transfusion. Experimentally prepared marrow-derived small lymphocytes behaved in sedimentation studies and after transfusion like a pure population of the lightly labeled small lymphocytes in normal lymph. Artificially reconstituted mixtures of marrow-derived and thymus-derived lymphocytes were qualitatively indistinguishable from normal lymphocyte populations.
Similar articles
- The life-span and recirculation of marrow-derived small lymphocytes from the rat thoracic duct.
Howard JC. Howard JC. J Exp Med. 1972 Feb 1;135(2):185-99. doi: 10.1084/jem.135.2.185. J Exp Med. 1972. PMID: 5062171 Free PMC article. - Rabbit gut-associated lymphoid tissue. Major pathway for thoracic duct lymphocyte circulation.
Perey DY, Milne RW. Perey DY, et al. Lab Invest. 1975 Dec;33(6):678-86. Lab Invest. 1975. PMID: 1081631 - Lymphocytes and macrophages in the lymphomyleoid complex.
Yoffey JM. Yoffey JM. Adv Exp Med Biol. 1979;121B:127-44. doi: 10.1007/978-1-4684-8914-9_12. Adv Exp Med Biol. 1979. PMID: 397745 Review. No abstract available. - Lymphocyte migration and immune responses.
Ford WL. Ford WL. Prog Allergy. 1975;19:1-59. doi: 10.1159/000313381. Prog Allergy. 1975. PMID: 56007 Review. No abstract available.
Cited by
- Transplant Tolerance, Not Only Clonal Deletion.
Hall BM, Verma ND, Tran GT, Hodgkinson SJ. Hall BM, et al. Front Immunol. 2022 Apr 21;13:810798. doi: 10.3389/fimmu.2022.810798. eCollection 2022. Front Immunol. 2022. PMID: 35529847 Free PMC article. Review. - Germinal center structure and function: lessons from CD19.
Carter RH, Myers R. Carter RH, et al. Semin Immunol. 2008 Feb;20(1):43-8. doi: 10.1016/j.smim.2007.12.007. Epub 2008 Feb 19. Semin Immunol. 2008. PMID: 18243730 Free PMC article. Review. - The sense of place in the immune system.
Cahalan MD, Gutman GA. Cahalan MD, et al. Nat Immunol. 2006 Apr;7(4):329-32. doi: 10.1038/ni0406-329. Nat Immunol. 2006. PMID: 16550194 Free PMC article. Review. - B lymphocyte chemotaxis regulated in association with microanatomic localization, differentiation state, and B cell receptor engagement.
Bleul CC, Schultze JL, Springer TA. Bleul CC, et al. J Exp Med. 1998 Mar 2;187(5):753-62. doi: 10.1084/jem.187.5.753. J Exp Med. 1998. PMID: 9480985 Free PMC article.
References
- J Immunol. 1970 Apr;104(4):798-804 - PubMed
- Nat New Biol. 1971 Apr 28;230(17):267-70 - PubMed
- Clin Exp Immunol. 1971 May;8(5):663-84 - PubMed
- Science. 1971 Jul 9;173(3992):154-6 - PubMed
- Proc Natl Acad Sci U S A. 1970 Oct;67(2):991-7 - PubMed