Tissue culture of normal rat glomeruli. Isolation and morphological characterization of two homogeneous cell lines - PubMed (original) (raw)
. 1979 Jan-Mar;2(1):15-26.
- PMID: 511605
Tissue culture of normal rat glomeruli. Isolation and morphological characterization of two homogeneous cell lines
J B Foidart et al. Invest Cell Pathol. 1979 Jan-Mar.
Abstract
Glomeruli were isolated from rat kidneys using enzymatic digestion, sieving and differential centrifugation. They were cultured in RPMI medium containing 15 per cent decomplemented fetal bovine serum. Using 3H-thymidine, two peaks of cell division occurred: the first was observed after 2 days in culture and reached its maximum on day 6; the second peak, starting on day 12, reached its maximum on day 22. Two cell types were identified by phase contrast, immunofluorescent and transmission electron microscopy. The first peak contained epithelial cells until day 6, while the second contained smooth muscle-like (mesangial) cells on day 30. The difference in the growth capacity of epithelial and smooth muscle-like cells allows their separate isolation by performing subcultures on day 6 for the former cells and on day 30 for the latter.
Similar articles
- Rat glomerular epithelial cells in culture. Parietal or visceral epithelial origin?
Nørgaard JO. Nørgaard JO. Lab Invest. 1987 Sep;57(3):277-90. Lab Invest. 1987. PMID: 3626518 - Tissue culture of normal rat glomeruli: contractile activity of the cultured mesangial cells.
Mahieu PR, Foidart JB, Dubois CH, Dechenne CA, Deheneffe J. Mahieu PR, et al. Invest Cell Pathol. 1980 Apr-Jun;3(2):121-8. Invest Cell Pathol. 1980. PMID: 7380664 - [Isolation and characterization of cells from primary culture of rat kidney glomeruli].
Gabrilevskaia OV, Ivanov AA, Terekhov SM, Shilov EM. Gabrilevskaia OV, et al. Biull Eksp Biol Med. 1990 Jan;109(1):57-60. Biull Eksp Biol Med. 1990. PMID: 2110488 Russian. - Glomerular cell culture.
Striker GE, Striker LJ. Striker GE, et al. Lab Invest. 1985 Aug;53(2):122-31. Lab Invest. 1985. PMID: 3894792 Review. - Isolated glomeruli and cultured mesangial cells as in vitro models to study immunosuppressive agents.
Potier M, L'Azou B, Cambar J. Potier M, et al. Cell Biol Toxicol. 1996 Dec;12(4-6):263-70. doi: 10.1007/BF00438156. Cell Biol Toxicol. 1996. PMID: 9034619 Review.
Cited by
- Sialic acid supplementation ameliorates puromycin aminonucleoside nephrosis in rats.
Pawluczyk IZ, Najafabadi MG, Brown JR, Bevington A, Topham PS. Pawluczyk IZ, et al. Lab Invest. 2015 Sep;95(9):1019-28. doi: 10.1038/labinvest.2015.78. Epub 2015 Jun 29. Lab Invest. 2015. PMID: 26121320 - Oryeongsan suppressed high glucose-induced mesangial fibrosis.
Yoon JJ, Lee YJ, Lee SM, Kang DG, Lee HS. Yoon JJ, et al. BMC Complement Altern Med. 2015 Feb 22;15:30. doi: 10.1186/s12906-015-0542-6. BMC Complement Altern Med. 2015. PMID: 25880429 Free PMC article. - Reactivity of human anti-alpha-galactosyl IgG antibody with alpha(1-->3)-linked galactosyl epitopes exposed on basement membranes and on glomerular epithelial cells: an in vitro and in vivo study in the mouse.
Vecchi ML, Davin JC, Castronovo V, Foidart JM, Malaise M, Foidart JB, Dechene C, Sangiorgi GB, Mahieu P. Vecchi ML, et al. Clin Exp Immunol. 1989 Nov;78(2):271-7. Clin Exp Immunol. 1989. PMID: 12412761 Free PMC article. - Cultured rat mesangial cells contain smooth muscle alpha-actin not found in vivo.
Elger M, Drenckhahn D, Nobiling R, Mundel P, Kriz W. Elger M, et al. Am J Pathol. 1993 Feb;142(2):497-509. Am J Pathol. 1993. PMID: 8434645 Free PMC article. - Uptake of nanoparticles by rat glomerular mesangial cells in vivo and in vitro.
Manil L, Davin JC, Duchenne C, Kubiak C, Foidart J, Couvreur P, Mahieu P. Manil L, et al. Pharm Res. 1994 Aug;11(8):1160-5. doi: 10.1023/a:1018993000633. Pharm Res. 1994. PMID: 7971718